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Phosphatidylinositol 4-kinase and phosphatidylinositol 4-phosphate 5-kinase assays. 2013

Yang Ju Im, and Irena Brglez, and Catherine Dieck, and Imara Y Perera, and Wendy F Boss
Department of Plant Biology, North Carolina State University, Raleigh, NC, USA.

Inositol lipid kinases are perhaps the easiest and most straightforward enzymes in the phosphoinositide pathway to analyze. In addition to monitoring lipid kinase-specific activity, lipid kinase assays can be used to quantify the inositol lipids present in isolated membranes (Jones et al., Methods Mol Biol 462:75-88, 2009). The lipid kinase assays are based on the fact that the more negatively charged phosphorylated lipid products are readily separated from their lipid substrates by thin layer chromatography. We have summarized our current protocols and identified important considerations for working with inositol lipids including different methods for substrate delivery when using recombinant proteins.

UI MeSH Term Description Entries
D010944 Plants Multicellular, eukaryotic life forms of kingdom Plantae. Plants acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations. It is a non-taxonomical term most often referring to LAND PLANTS. In broad sense it includes RHODOPHYTA and GLAUCOPHYTA along with VIRIDIPLANTAE. Plant
D057075 Enzyme Assays Methods used to measure the relative activity of a specific enzyme or its concentration in solution. Typically an enzyme substrate is added to a buffer solution containing enzyme and the rate of conversion of substrate to product is measured under controlled conditions. Many classical enzymatic assay methods involve the use of synthetic colorimetric substrates and measuring the reaction rates using a spectrophotometer. Enzymatic Assays,Indirect Enzymatic Assays,Indirect Enzyme Assays,Assay, Enzymatic,Assay, Enzyme,Assay, Indirect Enzymatic,Assay, Indirect Enzyme,Assays, Enzymatic,Assays, Enzyme,Assays, Indirect Enzymatic,Assays, Indirect Enzyme,Enzymatic Assay,Enzymatic Assay, Indirect,Enzymatic Assays, Indirect,Enzyme Assay,Enzyme Assay, Indirect,Enzyme Assays, Indirect,Indirect Enzymatic Assay,Indirect Enzyme Assay
D017853 Phosphotransferases (Alcohol Group Acceptor) A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.
D019870 1-Phosphatidylinositol 4-Kinase An enzyme that catalyzes the conversion of phosphatidylinositol (PHOSPHATIDYLINOSITOLS) to phosphatidylinositol 4-phosphate, the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Phosphatidylinositol 4-Kinase,PtdIns 4-Kinase,PI 4-Kinase,Phosphatidylinositiol Kinase,Phosphatidylinositol Kinase Type II,Phosphoinositide Kinase,1 Phosphatidylinositol 4 Kinase,Kinase, Phosphatidylinositiol,Kinase, Phosphoinositide,PI 4 Kinase,Phosphatidylinositol 4 Kinase,PtdIns 4 Kinase

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