BIOMAT Database Logo BIOMAT Database Logo

Histamine transport and metabolism are deranged in salivary glands in Sjogren's syndrome. 2013

Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Department of Medicine, Institute of Clinical Medicine, Finland.

OBJECTIVE To study histamine transport and metabolism of salivary gland (SG) epithelial cells in healthy controls and SS patients. METHODS Enzymes and transporters involved in histamine metabolism were analysed in cultured human submandibular salivary gland (HSG) epithelial cells and tissue sections using quantitative real-time PCR and immunostaining. HSG cells were used to study [(3)H]histamine uptake [(±1-methyl-4-phenylpyridinium (MPP)] and efflux by liquid scintillation counting. RESULTS mRNA levels of l-histidine decarboxylase (HDC) and histamine-N-methyltransferase (HNMT) were similar in the control and SS glands, but diamine oxidase was not expressed at all. Organic cation transporter 3 (OCT3) in healthy SG was localized in the acinar and ductal cells, whereas OCT2 was restricted to the myoepithelial cells. Both transporters were significantly decreased in SS at mRNA and protein levels. OCT3-mRNA levels in HSG cells were significantly higher than those of the other studied transporters. Uptake of [(3)H]histamine was inhibited by MPP in a time-dependent manner, whereas [(3)H]histamine-preloaded HSG cells released it. CONCLUSIONS Ductal epithelial cells are non-professional histamine-producing cells able to release histamine via OCTs at the resting state up to ∼100 nM, enough to excite H3R/H4R(+) epithelial cells, but not H1R, which requires burst release from mast cells. At the stimulated phase, 50-60 μM histamine passes from the interstitial fluid through the acinar cells to saliva, whereas uptake by ductal cells leads to intracellular degradation by HNMT. OCT3/histamine/H4R-mediated cell maintenance and down-regulation of high histamine levels fail in SS SGs.

UI MeSH Term Description Entries
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D006632 Histamine An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter. Ceplene,Histamine Dihydrochloride,Histamine Hydrochloride,Peremin
D006637 Histamine N-Methyltransferase An enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine to histamine, forming N-methylhistamine, the major metabolite of histamine in man. EC 2.1.1.8. Histamine Methyltransferase,Histamine N Methyltransferase,Methyltransferase, Histamine,N-Methyltransferase, Histamine
D006640 Histidine Decarboxylase An enzyme that catalyzes the decarboxylation of histidine to histamine and carbon dioxide. It requires pyridoxal phosphate in animal tissues, but not in microorganisms. EC 4.1.1.22. Histidine Carboxy-Lyase,Carboxy-Lyase, Histidine,Decarboxylase, Histidine,Histidine Carboxy Lyase
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000075102 Organic Cation Transporter 2 Organic cation transporter consisting of twelve transmembrane domains and expressed primarily in the kidney. It transports a wide range of metabolites, drugs, and neurotransmitters from the blood to the KIDNEY TUBULES, including DOPAMINE; SEROTONIN; CHOLINE; and CISPLATIN. OCT2 Protein,SLC22A2 Protein,Solute Carrier Family 22 Member 2
D001692 Biological Transport The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments. Transport, Biological,Biologic Transport,Transport, Biologic
D012859 Sjogren's Syndrome Chronic inflammatory and autoimmune disease in which the salivary and lacrimal glands undergo progressive destruction by lymphocytes and plasma cells resulting in decreased production of saliva and tears. The primary form, often called sicca syndrome, involves both KERATOCONJUNCTIVITIS SICCA and XEROSTOMIA. The secondary form includes, in addition, the presence of a connective tissue disease, usually rheumatoid arthritis. Sicca Syndrome,Sjogren Syndrome,Sjogrens Syndrome,Syndrome, Sicca,Syndrome, Sjogren's
D013363 Submandibular Gland One of two salivary glands in the neck, located in the space bound by the two bellies of the digastric muscle and the angle of the mandible. It discharges through the submandibular duct. The secretory units are predominantly serous although a few mucous alveoli, some with serous demilunes, occur. (Stedman, 25th ed) Submaxillary Gland,Gland, Submandibular,Gland, Submaxillary,Glands, Submandibular,Glands, Submaxillary,Submandibular Glands,Submaxillary Glands

Related Publications

Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
[Salivary glands and sjogren's syndrome].
April 1977, Den Norske tannlaegeforenings tidende,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Histamine metabolism and transport are deranged in human keratinocytes in oral lichen planus.
May 2017, The British journal of dermatology,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
[Sjogren's syndrome and changes in the salivary glands].
October 1969, Deutsche Zahn-, Mund-, und Kieferheilkunde mit Zentralblatt fur die gesamte Zahn-, Mund-, und Kieferheilkunde,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Androgens and integrins in salivary glands in Sjogren's syndrome.
June 2010, The Journal of rheumatology,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Ultrasonographic damages of major salivary glands are associated with cryoglobulinemic vasculitis and lymphoma in primary Sjogren's syndrome: are the ultrasonographic features of the salivary glands new prognostic markers in Sjogren's syndrome?
July 2021, Annals of the rheumatic diseases,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
[Scintigraphy of salivary glands in patients with Sjogren's syndrome].
January 1978, Reumatologia,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Quantitative isotope diagnostic method of the salivary glands in Sjogren's syndrome.
January 1973, Dento maxillo facial radiology,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Response to: 'Ultrasonographic damages of major salivary glands are associated with cryoglobulinemic vasculitis and lymphoma in primary Sjogren's syndrome: are the ultrasonographic features of the salivary glands new prognostic markers in Sjogren's syndrome?' by Coiffier et al.
July 2021, Annals of the rheumatic diseases,
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Ectopic germinal centers are rare in Sjogren's syndrome salivary glands and do not exclude autoreactive B cells.
March 2009, Journal of immunology (Baltimore, Md. : 1950),
Vasily Stegaev, and Anne T Nies, and Pauliina Porola, and Diana Mieliauskaite, and Francisca Sánchez-Jiménez, and Jóse L Urdiales, and Tarvo Sillat, and Hubert G Schwelberger, and Paul L Chazot, and Mwape Katebe, and Zygmunt Mackiewicz, and Yrjö T Konttinen, and Dan C E Nordström
Ultrasonographic Evaluation of Salivary Glands for Sjogren's Syndrome: Diagnostic and Monitoring Insights.
October 2023, Journal of visualized experiments : JoVE,
Copied contents to your clipboard!