Expression, crystallization and preliminary crystallographic study of GluB from Corynebacterium glutamicum. 2013

Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.

GluB is a substrate-binding protein (SBP) which participates in the uptake of glutamic acid in Corynebacterium glutamicum, a Gram-positive bacterium. It is part of an ATP-binding cassette (ABC) transporter system. Together with the transmembrane proteins GluC and GluD and the cytoplasmic protein GluA, which couples the hydrolysis of ATP to the translocation of glutamate, they form a highly active glutamate-uptake system. As part of efforts to study the amino-acid metabolism, especially the metabolism of glutamic acid by C. glutamicum, a bacterium that is widely used in the industrial production of glutamic acid, the GluB protein was expressed, purified and crystallized, an X-ray diffraction data set was collected to a resolution of 1.9 Å and preliminary crystallographic analysis was performed. The crystal belonged to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 82.50, c = 72.69 Å.

UI MeSH Term Description Entries
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D015964 Gene Expression Regulation, Bacterial Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria. Bacterial Gene Expression Regulation,Regulation of Gene Expression, Bacterial,Regulation, Gene Expression, Bacterial
D048230 Corynebacterium glutamicum A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.
D018360 Crystallography, X-Ray The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) X-Ray Crystallography,Crystallography, X Ray,Crystallography, Xray,X Ray Crystallography,Xray Crystallography,Crystallographies, X Ray,X Ray Crystallographies
D018528 ATP-Binding Cassette Transporters A family of MEMBRANE TRANSPORT PROTEINS that require ATP hydrolysis for the transport of substrates across membranes. The protein family derives its name from the ATP-binding domain found on the protein. ABC Transporter,ABC Transporters,ATP-Binding Cassette Transporter,ATP Binding Cassette Transporter,ATP Binding Cassette Transporters,Cassette Transporter, ATP-Binding,Transporter, ABC,Transporter, ATP-Binding Cassette,Transporters, ABC,Transporters, ATP-Binding Cassette
D033902 Periplasmic Binding Proteins Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane. Periplasmic Binding Protein,Binding Protein, Periplasmic,Binding Proteins, Periplasmic,Protein, Periplasmic Binding

Related Publications

Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
September 2013, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
September 2003, Acta crystallographica. Section D, Biological crystallography,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
August 2011, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
November 2009, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
January 2009, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
November 2007, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
February 2007, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
July 2006, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
August 2009, Acta crystallographica. Section F, Structural biology and crystallization communications,
Qingbo Liu, and Defeng Li, and Yonglin Hu, and Da Cheng Wang
August 1996, Proteins,
Copied contents to your clipboard!