There are many diverse methods for the assessment of Eustachian tube (ET) function. Clinical studies have demonstrated good results for several clinical tests, tympanometry and a number of manometric approaches, such as the nine-step test. The relevance of tubomanometry according to Estève requires further investigation. MRI studies in particular have considerably enhanced our knowledge of ET function. Endoscopy and conventional Schüller radiography are routinely employed in the clinic. The pressure chamber is ideal for simulating the most diverse test conditions, but it is only used experimentally. Sonotubometry has both the potential and the necessary level of technical advancement for a broader clinical application. The majority of investigations have demonstrated that there is no single valid method. There is currently no technique available that is capable of reflecting all aspects of ET function. Although several methods have found clinical application, a commonly accepted golden standard for ET testing still has to be established. Further clinical studies are necessary to generate an adequate amount of data pertaining to the various ET function tests.