Biomaterial Database

Monoclonal antibodies reacting with tryptic hyaluronic acid-binding region and link protein fragments of bovine nasal cartilage proteoglycan. 1990

H D Keiser

Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.

BALB/c mice were immunized with isolated trypsin-produced hyaluronic acid-binding region (HABR) or HABR/link protein complex from bovine nasal cartilage proteoglycan (PG) aggregates conjugated to keyhole limpet hemocyanin. The monoclonal antibodies (Mabs) raised were characterized by solid-phase ELISA inhibition and SDS-polyacrylamide gel electrophoresis immunoblotting. Nine Mabs react with intact PG monomer, HABR and HABR/link complex. Two of the anti-HABR Mabs appear to be directed to epitopes in or near the HA-binding site of PG monomer; one of these epitopes is insensitive to reduction and alkylation and pronase treatment and is likely to consist of carbohydrate. The remaining anti-HABR Mabs react with PG aggregate, are non-reactive with pronase-treated PG monomer and vary in their reactivity with reduced and alkylated PG monomer. Three Mabs react with link protein-related epitopes. One of these Mabs reacts only with the tryptic link protein fragment, the others also react with PG aggregates and the two native link proteins. Immunological studies of cartilage PG should be facilitated by the availability of Mabs specific for these functionally significant components in native PG aggregates.

UI	MeSH Term	Description	Entries
D009300	Nasal Septum	The partition separating the two NASAL CAVITIES in the midplane. It is formed by the SEPTAL NASAL CARTILAGE, parts of skull bones (ETHMOID BONE; VOMER), and membranous parts.	Nasal Septums,Septum, Nasal,Septums, Nasal
D011509	Proteoglycans	Glycoproteins which have a very high polysaccharide content.	Proteoglycan,Proteoglycan Type H
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D006820	Hyaluronic Acid	A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.	Amo Vitrax,Amvisc,Biolon,Etamucine,Healon,Hyaluronan,Hyaluronate Sodium,Hyvisc,Luronit,Sodium Hyaluronate,Acid, Hyaluronic,Hyaluronate, Sodium,Vitrax, Amo
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal