Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library. 2013

Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. yongliqin119@163.com

The NS2 protein of Bluetongue virus (BTV) is an important non-structural protein and plays important roles in viral replication and assembly. In this study, one monoclonal antibody (mAb), 4D4, was raised against BTV8 NS2. Phage display technology was used and identified the consensus binding motif SNYD recognized by mAb 4D4. To define the minimal region required for antibody binding, a panel of synthetic peptides encompassing SNYD derived from the BTV8 NS2 was then used to more specifically define the 4D4 epitope as (149)RSNYDV(154). Furthermore, amino acid sequence alignments of different BTV serotypes and other orbiviruses suggested that this epitope is highly conserved among the BTV serotypes. The mAb reagent generated in this study may be applied to the development of BTV diagnosis and surveillance programs and the epitope defined here can lead to important insights into how BTV might interact with the sheep's immune system.

UI MeSH Term Description Entries
D001820 Bluetongue virus The type species of ORBIVIRUS causing a serious disease in sheep, especially lambs. It may also infect wild ruminants and other domestic animals. Ovine Catarrhal Fever Virus,Blue Tongue Virus,Blue Tongue Viruses,Bluetongue Viruses
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006224 Cricetinae A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS. Cricetus,Hamsters,Hamster
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016415 Sequence Alignment The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms. Sequence Homology Determination,Determination, Sequence Homology,Alignment, Sequence,Alignments, Sequence,Determinations, Sequence Homology,Sequence Alignments,Sequence Homology Determinations
D017361 Viral Nonstructural Proteins Proteins encoded by a VIRAL GENOME that are not structural components of VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY. Nonstructural Proteins, Viral,NS Proteins, Viral,Viral NS Proteins,Viral Non-Structural Proteins,Viral Nonstructural Protein,Viral Nonstructural Proteins NS1,Viral Nonstructural Proteins NS2,Nonstructural Protein, Viral,Viral Non Structural Proteins
D018985 Epitopes, B-Lymphocyte Antigenic determinants recognized and bound by the B-cell receptor. Epitopes recognized by the B-cell receptor are located on the surface of the antigen. B-Cell Epitopes,B-Lymphocyte Epitopes,B-Cell Epitope,B-Lymphocyte Epitope,B Cell Epitope,B Cell Epitopes,B Lymphocyte Epitope,B Lymphocyte Epitopes,Epitope, B-Cell,Epitope, B-Lymphocyte,Epitopes, B Lymphocyte,Epitopes, B-Cell
D019151 Peptide Library A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide. Phage Display Peptide Library,Random Peptide Library,Peptide Phage Display Library,Phage Display Library, Peptide,Synthetic Peptide Combinatorial Library,Synthetic Peptide Library,Libraries, Peptide,Libraries, Random Peptide,Libraries, Synthetic Peptide,Library, Peptide,Library, Random Peptide,Library, Synthetic Peptide,Peptide Libraries,Peptide Libraries, Random,Peptide Libraries, Synthetic,Peptide Library, Random,Peptide Library, Synthetic,Random Peptide Libraries,Synthetic Peptide Libraries

Related Publications

Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
January 2015, PloS one,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
January 2012, PloS one,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
March 1995, FEMS microbiology letters,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
January 2005, Journal of medical virology,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
April 1995, Journal of protein chemistry,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
January 2018, PloS one,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
August 2008, Virus research,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
December 1998, The Journal of general virology,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
May 2006, Acta biochimica et biophysica Sinica,
Yong-Li Qin, and En-Cheng Sun, and Ni-Hong Liu, and Tao Yang, and Qing-Yuan Xu, and Jing Zhao, and Wen-Shi Wang, and Peng Wei, and Yu-Fei Feng, and Jun-Ping Li, and Dong-Lai Wu
January 1998, Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica,
Copied contents to your clipboard!