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In vitro fertilization in the domestic fowl (Gallus domesticus) was investigated by observation of the early nuclear events. Ova retrieved from the fimbria following ovulation were inseminated in vitro with 10(6)-10(7) spermatozoa in Dulbecco's modified Eagle's medium (DMEM) for 10 min and then further incubated in DMEM + albumen for 1, 2, 3, or 4 hr. These eggs were histologically examined by epifluorescent microscopy after staining with 4',6'-diamidino-2-phenylindole (DAPI). Nuclei of spermatozoa at various stages of transformation were observed in the ova incubated for 1-3 hr. Close pairing of two pronuclei, presumed to be male and female juxtaposition, was detected in ova incubated for 4 hr. These data provide direct evidence for the in vitro fertilization of fowl eggs and suggested that the early process of in vitro fertilization is comparable to that of in vivo fertilization.
A Nakanishi, and
K Utsumi, and
A Iritani
February 1987,
Journal of anatomy,
A Nakanishi, and
K Utsumi, and
A Iritani
June 2013,
Reproduction in domestic animals = Zuchthygiene,
A Nakanishi, and
K Utsumi, and
A Iritani
April 1969,
Avian diseases,
A Nakanishi, and
K Utsumi, and
A Iritani
August 1982,
Kidney international. Supplement,
A Nakanishi, and
K Utsumi, and
A Iritani
January 1967,
Sbornik vedeckych praci Lekarske fakulty Karlovy university v Hradci Kralove,
A Nakanishi, and
K Utsumi, and
A Iritani
May 1983,
Journal of anatomy,
A Nakanishi, and
K Utsumi, and
A Iritani
December 1974,
The Journal of endocrinology,
A Nakanishi, and
K Utsumi, and
A Iritani
May 1979,
Research in veterinary science,
A Nakanishi, and
K Utsumi, and
A Iritani
January 1984,
The Journal of endocrinology,
A Nakanishi, and
K Utsumi, and
A Iritani
May 1975,
Comparative biochemistry and physiology. A, Comparative physiology,
