X-ray microtomographic visualization of Escherichia coli by metalloprotein overexpression. 2013

Ryuta Mizutani, and Keisuke Taguchi, and Masato Ohtsuka, and Minoru Kimura, and Akihisa Takeuchi, and Kentaro Uesugi, and Yoshio Suzuki
Graduate School of Engineering, Tokai University, Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan. ryuta@tokai-u.jp

This paper reports X-ray microtomographic visualization of the microorganism Escherichia coli overexpressing a metalloprotein ferritin. The three-dimensional distribution of linear absorption coefficients determined using a synchrotron radiation microtomograph with a simple projection geometry revealed that the X-ray absorption was homogeneously distributed, suggesting that every E. coli cell was labeled with the ferritin. The ferritin-expressing E. coli exhibited linear absorption coefficients comparable with those of phosphotungstic acid stained cells. The submicrometer structure of the ferritin-expressing E. coli cells was visualized by Zernike phase contrast using an imaging microtomograph equipped with a Fresnel zone plate. The obtained images revealed curved columnar or bunching oval structures corresponding to the E. coli cells. These results indicate that the metalloprotein overexpression facilitates X-ray visualization of three-dimensional cellular structures of biological objects.

UI MeSH Term Description Entries
D008667 Metalloproteins Proteins that have one or more tightly bound metal ions forming part of their structure. (Dorland, 28th ed) Metalloprotein
D004577 Electron Probe Microanalysis Identification and measurement of ELEMENTS and their location based on the fact that X-RAYS emitted by an element excited by an electron beam have a wavelength characteristic of that element and an intensity related to its concentration. It is performed with an electron microscope fitted with an x-ray spectrometer, in scanning or transmission mode. Microscopy, Electron, X-Ray Microanalysis,Spectrometry, X-Ray Emission, Electron Microscopic,Spectrometry, X-Ray Emission, Electron Probe,X-Ray Emission Spectrometry, Electron Microscopic,X-Ray Emission Spectrometry, Electron Probe,X-Ray Microanalysis, Electron Microscopic,X-Ray Microanalysis, Electron Probe,Microanalysis, Electron Probe,Spectrometry, X Ray Emission, Electron Microscopic,Spectrometry, X Ray Emission, Electron Probe,X Ray Emission Spectrometry, Electron Microscopic,X Ray Emission Spectrometry, Electron Probe,X-Ray Microanalysis,Electron Probe Microanalyses,Microanalyses, Electron Probe,Microanalysis, X-Ray,Probe Microanalyses, Electron,Probe Microanalysis, Electron,X Ray Microanalysis,X Ray Microanalysis, Electron Microscopic,X Ray Microanalysis, Electron Probe
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D055114 X-Ray Microtomography X-RAY COMPUTERIZED TOMOGRAPHY with resolution in the micrometer range. MicroCT,Microcomputed Tomography,X-Ray Micro-CAT Scans,X-Ray Micro-CT,X-Ray Micro-CT Scans,X-Ray Micro-Computed Tomography,X-Ray Microcomputed Tomography,X-ray MicroCT,Xray Micro-CT,Xray MicroCT,Micro-CAT Scan, X-Ray,Micro-CAT Scans, X-Ray,Micro-CT Scan, X-Ray,Micro-CT Scans, X-Ray,Micro-CT, X-Ray,Micro-CT, Xray,Micro-CTs, X-Ray,Micro-CTs, Xray,Micro-Computed Tomography, X-Ray,MicroCT, X-ray,MicroCT, Xray,MicroCTs,MicroCTs, X-ray,MicroCTs, Xray,Microcomputed Tomography, X-Ray,Microtomography, X-Ray,Scan, X-Ray Micro-CAT,Scan, X-Ray Micro-CT,Scans, X-Ray Micro-CAT,Scans, X-Ray Micro-CT,Tomography, Microcomputed,Tomography, X-Ray Micro-Computed,Tomography, X-Ray Microcomputed,X Ray Micro CAT Scans,X Ray Micro CT,X Ray Micro CT Scans,X Ray Micro Computed Tomography,X Ray Microcomputed Tomography,X Ray Microtomography,X ray MicroCT,X-Ray Micro-CAT Scan,X-Ray Micro-CT Scan,X-Ray Micro-CTs,X-ray MicroCTs,Xray Micro CT,Xray Micro-CTs,Xray MicroCTs
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide

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