Comparative study of colorimetric DNA hybridization method and conventional culture procedure for detection of Salmonella in foods. 1990

S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
Gene-Trak Systems, Framingham, MA 01701.

A second generation nucleic acid hybridization assay has been developed and evaluated against the conventional culture method for detection of salmonellae in foods. The assay involves a liquid hybridization with Salmonella-specific oligonucleotide probes, capture of probe:target hybrids onto a solid support (plastic dipstick), and a colorimetric end point detection. The assay can be completed in 2.5 h, following approximately 44 h of culture enrichment. One thousand samples representing 20 food types were analyzed in parallel by both methods. Samples included uninoculated test product, and product inoculated with Salmonella at 2 levels. Eighteen Salmonella serotypes were used as inocula. The data demonstrate that the colorimetric hybridization method and the conventional culture method are equivalent in their ability to detect Salmonella contamination of foods.

UI MeSH Term Description Entries
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D003124 Colorimetry Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012475 Salmonella A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.

Related Publications

S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
October 1995, Journal of food protection,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
May 1987, Journal of food protection,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
April 1991, Journal of food protection,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
January 1987, Journal - Association of Official Analytical Chemists,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
January 1990, Journal - Association of Official Analytical Chemists,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
November 1983, Applied and environmental microbiology,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
May 1987, Journal of food protection,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
January 1990, Journal - Association of Official Analytical Chemists,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
April 1984, Nucleic acids research,
S W Chan, and S G Wilson, and M Vera-Garcia, and K Whippie, and M Ottaviani, and A Whilby, and A Shah, and A Johnson, and M A Mozola, and D N Halbert
January 1993, Journal of AOAC International,
Copied contents to your clipboard!