Goniothalamin selectively induces apoptosis on human hepatoblastoma cells through caspase-3 activation. 2013

Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
a Department of Cell and Molecular Biology , Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia (UPM) , 43400 Serdang, Selangor , Malaysia.

Goniothalamin is a biologically active styrylpyrone derivative isolated from various Goniothalamus species. The ability of goniothalamin to induce apoptosis via caspase-3 activation against hepatoblastoma (HepG2) and normal liver cells (Chang cells) was studied using morphological and biochemical evaluations. HepG2 and Chang cells were treated with goniothalamin for 72 h and analysed by TUNEL and Annexin-V/PI staining. Furthermore, the post-mitochondrial caspase-3 was quantified using ELISA. In view of our results, goniothalamin induced apoptosis on treated cells via alteration of cellular membrane integrity and cleavage of DNA. On the other hand, post-mitochondrial caspase-3 activity was significantly elevated in HepG2 cells treated with goniothalamin after 72 h. These findings suggest that goniothalamin induced apoptosis on HepG2 liver cancer cells via induction of caspase-3 with less sensitivity on the cell line of Chang cells.

UI MeSH Term Description Entries
D008113 Liver Neoplasms Tumors or cancer of the LIVER. Cancer of Liver,Hepatic Cancer,Liver Cancer,Cancer of the Liver,Cancer, Hepatocellular,Hepatic Neoplasms,Hepatocellular Cancer,Neoplasms, Hepatic,Neoplasms, Liver,Cancer, Hepatic,Cancer, Liver,Cancers, Hepatic,Cancers, Hepatocellular,Cancers, Liver,Hepatic Cancers,Hepatic Neoplasm,Hepatocellular Cancers,Liver Cancers,Liver Neoplasm,Neoplasm, Hepatic,Neoplasm, Liver
D011753 Pyrones Keto-pyrans.
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004789 Enzyme Activation Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme. Activation, Enzyme,Activations, Enzyme,Enzyme Activations
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D017209 Apoptosis A regulated cell death mechanism characterized by distinctive morphologic changes in the nucleus and cytoplasm, including the endonucleolytic cleavage of genomic DNA, at regularly spaced, internucleosomal sites, i.e., DNA FRAGMENTATION. It is genetically programmed and serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. Apoptosis, Extrinsic Pathway,Apoptosis, Intrinsic Pathway,Caspase-Dependent Apoptosis,Classic Apoptosis,Classical Apoptosis,Programmed Cell Death,Programmed Cell Death, Type I,Apoptoses, Extrinsic Pathway,Apoptoses, Intrinsic Pathway,Apoptosis, Caspase-Dependent,Apoptosis, Classic,Apoptosis, Classical,Caspase Dependent Apoptosis,Cell Death, Programmed,Classic Apoptoses,Extrinsic Pathway Apoptoses,Extrinsic Pathway Apoptosis,Intrinsic Pathway Apoptoses,Intrinsic Pathway Apoptosis
D053148 Caspase 3 A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA. CASP3,Apopain,Caspase-3,Pro-Caspase-3,Procaspase-3,Pro Caspase 3,Procaspase 3
D056945 Hep G2 Cells A human liver tumor cell line used to study a variety of liver-specific metabolic functions. Cell Line, Hep G2,Cell Line, Hepatoblastoma G2,Hep G2 Cell Line,HepG2 Cells,Hepatoblastoma G2 Cell Line,Cell, Hep G2,Cell, HepG2,Cells, Hep G2,Cells, HepG2,Hep G2 Cell,HepG2 Cell

Related Publications

Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
May 2016, Oncology reports,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
August 2010, Toxicological sciences : an official journal of the Society of Toxicology,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
December 2013, Cell death & disease,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
August 2009, Blood,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
December 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
January 2007, The American journal of Chinese medicine,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
August 2006, Journal of veterinary medicine. B, Infectious diseases and veterinary public health,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
January 2016, European journal of gynaecological oncology,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
September 2009, Journal of agricultural and food chemistry,
Mothanna Al-Qubaisi, and Rozita Rosli, and Tamilselvan Subramani, and Abdul Rahman Omar, and Swee Keong Yeap, and Abdul Manaf Ali, and Noorjahan Banu Alitheen
September 2011, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Copied contents to your clipboard!