Biomaterial Database

[Preparation of monoclonal antibodies against human bone gamma-carboxyglutamic acid containing protein (BGP) and its immunohistochemical application to various human tissues]. 1990

J Takada, and T Ohta, and H Koshiba

Department of Orthopaedic Surgery, and Pathology, Sapporo Medical College, Japan.

Bone gamma-carboxyglutamic acid containing protein (BGP) has been considered to play an important role in mineralization of bone, because this protein is capable of binding to calcium ions. However, the function and localization of BGP in normal bones have not yet been fully understood. In this study, we prepared monoclonal antibodies against human BGP, by immunizing BALB/c mice with human BGP. All five monoclonal antibodies obtained were shown to bind specifically to human BGP, but not to the other bone-derived proteins. In immunohistochemical analysis, these antibodies were shown to react with osteoblasts and osteocytes in normal human bone tissues, but not with chondrocytes, osteoclasts and osteoid tissues. These antibodies are expected to be useful for the diagnosis of human osteoblastic tumors and also for the analysis of the role of BGP in the ossification process in bone.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D010017	Osteoma, Osteoid	A benign osteoblastic tumor with central vascularized nidus surrounded by normal reactive bone. It occurs especially in second decade of life most commonly in the femoral neck but can occur in any bone and any site within a bone.	Osteoid Osteoma,Osteoid Osteomas,Osteomas, Osteoid
D001859	Bone Neoplasms	Tumors or cancer located in bone tissue or specific BONES.	Bone Cancer,Cancer of Bone,Cancer of the Bone,Neoplasms, Bone,Bone Neoplasm,Neoplasm, Bone
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D015055	1-Carboxyglutamic Acid	Found in various tissues, particularly in four blood-clotting proteins including prothrombin, in kidney protein, in bone protein, and in the protein present in various ectopic calcifications.	gamma-Carboxyglutamate,gamma-Carboxyglutamic Acid,3-Amino-1,1,3-propanetricarboxylic Acid,1 Carboxyglutamic Acid,gamma Carboxyglutamate,gamma Carboxyglutamic Acid
D015675	Osteocalcin	Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.	Bone Gla Protein,Calcium-Binding Protein, Vitamin K-Dependent,Gla Protein, Bone,Vitamin K-Dependent Bone Protein,4-Carboxyglutamic Protein, Bone,Bone gamma-Carboxyglutamic Acid Protein,4 Carboxyglutamic Protein, Bone,Bone 4-Carboxyglutamic Protein,Bone gamma Carboxyglutamic Acid Protein,Calcium Binding Protein, Vitamin K Dependent,Protein, Bone 4-Carboxyglutamic,Protein, Bone Gla,Vitamin K Dependent Bone Protein
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus