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Human, rat and bovine fibrinogen were radioiodinated using a modified chloramine-T method under mild labelling conditions and with a satisfactory labelling efficiency. The 125I-human, rat and bovine fibrinogen preparations obtained met the requirements with respect to clottability, low content of 125Iodide as well as stability in vitro and in vivo. SDS-PAGE mobility demonstrated that the integrity of the fibrinogen molecule was not affected by the labelling procedure.
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