Biomaterial Database

First genetic detection of Coxiella burnetii in Zambian livestock. 2013

Yongjin Qiu, and Ryo Nakao, and Boniface Namangala, and Chihiro Sugimoto

Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan. yongjin_qiu@czc.hokudai.ac.jp

Q fever is a widespread zoonosis caused by Coxiella burnetii, an obligate intracellular gram-negative bacterium. The investigation of C. burnetii infection in Zambian livestock was carried out using molecular detection techniques. A total of 489 cattle and 53 goat blood samples were collected from Chama, Chongwe, Monze, and Petauke districts in Zambia. Molecular screening by polymerase chain reaction was performed using C. burnetii-species-specific primers. In total, 38 cattle and 4 goat samples were positive. The prevalence of C. burnetii differed among the four sites, with Chama (Eastern province) recording the highest, although Monze (Southern province) did not record any case of the bacteria. This study reports the first genetic detection of C. burnetii in Zambia.

UI	MeSH Term	Description	Entries
D011778	Q Fever	An acute infectious disease caused by COXIELLA BURNETII. It is characterized by a sudden onset of FEVER; HEADACHE; malaise; and weakness. In humans, it is commonly contracted by inhalation of infected dusts derived from infected domestic animals (ANIMALS, DOMESTIC).	Coxiella burnetii Fever,Query Fever,Acute Q Fever,Chronic Q Fever,Coxiella burnetii Infection,Coxiella burnetii Vector-Borne Disease,Acute Q Fevers,Chronic Q Fevers,Coxiella burnetii Fevers,Coxiella burnetii Infections,Coxiella burnetii Vector Borne Disease,Fever, Acute Q,Fever, Chronic Q,Fever, Coxiella burnetii,Fever, Q,Fever, Query,Fevers, Acute Q,Fevers, Chronic Q,Fevers, Coxiella burnetii,Fevers, Q,Fevers, Query,Infection, Coxiella burnetii,Infections, Coxiella burnetii,Q Fever, Acute,Q Fever, Chronic,Q Fevers,Q Fevers, Acute,Q Fevers, Chronic,Query Fevers
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D004269	DNA, Bacterial	Deoxyribonucleic acid that makes up the genetic material of bacteria.	Bacterial DNA
D006041	Goats	Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.	Capra,Capras,Goat
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015024	Zambia	A republic in southern Africa, south of DEMOCRATIC REPUBLIC OF THE CONGO and TANZANIA, and north of ZIMBABWE. Its capital is Lusaka. It was formerly called Northern Rhodesia.	Northern Rhodesia,Rhodesia, Northern,Republic of Zambia
D015047	Zoonoses	Diseases of non-human animals that may be transmitted to HUMANS or may be transmitted from humans to non-human animals.	Zoonotic Spillover,Zoonotic Diseases,Zoonotic Infections,Zoonotic Infectious Diseases,Disease, Zoonotic,Disease, Zoonotic Infectious,Diseases, Zoonotic,Diseases, Zoonotic Infectious,Infection, Zoonotic,Infections, Zoonotic,Infectious Disease, Zoonotic,Infectious Diseases, Zoonotic,Spillovers, Zoonotic,Zoonotic Disease,Zoonotic Infection,Zoonotic Infectious Disease,Zoonotic Spillovers
D015995	Prevalence	The total number of cases of a given disease in a specified population at a designated time. It is differentiated from INCIDENCE, which refers to the number of new cases in the population at a given time.	Period Prevalence,Point Prevalence,Period Prevalences,Point Prevalences,Prevalence, Period,Prevalence, Point,Prevalences
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D016997	Coxiella burnetii	A species of gram-negative bacteria that grows preferentially in the vacuoles of the host cell. It is the etiological agent of Q FEVER.