Determinative and confirmatory method for residues of tetracyclines in honey by LC-MS/MS. 2013

Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
a State General Laboratory of Cyprus , Veterinary Drug Residues Laboratory , Nicosia , Cyprus.

A limited number of substances are authorised for the treatment of bees. Maximum residue limits (MRLs) are set for tetracyclines in several matrices, but not for honey. Nevertheless, tetracycline antibiotics may be used in order to prevent bacterial diseases and the loss of honey bee populations. In this study, a sensitive multi-residue LC-MS/MS method was developed and optimised for the quantitative and qualitative determination of tetracycline residues in honey. Homogenisation of samples under acidic conditions was performed and solid-phase extraction was carried out. The eluate was evaporated under nitrogen and dissolved in an aqueous methanol solution prior to filtration. A mobile phase composed of acetic acid-water and acetic acid-acetonitrile was used. Separation of tetracycline, oxytetracycline, chlortetracycline and doxytetracycline was achieved by using gradient elution on a C18 chromatography column. The analytical method was validated according to Commission Decision 2002/657/EC by the analysis of spiked samples around the recommended concentration of 20 μg kg(-1) by EURL Guidance Paper, December 2007. A matrix effect was observed, so quantification was based on an external matrix calibration curve. Calculated decision limits (CCα) were lower than 10 μg kg(-1) for all tetracyclines. Good linearity, repeatability and within-laboratory reproducibility were achieved.

UI MeSH Term Description Entries
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D004350 Drug Residues Drugs and their metabolites which are found in the edible tissues and milk of animals after their medication with specific drugs. This term can also apply to drugs found in adipose tissue of humans after drug treatment. Drug Residue,Residue, Drug,Residues, Drug
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D006722 Honey A sweet viscous liquid food, produced in the honey sacs of various bees from nectar collected from flowers. The nectar is ripened into honey by inversion of its sucrose sugar into fructose and glucose. It is somewhat acidic and has mild antiseptic properties, being sometimes used in the treatment of burns and lacerations. Honeys
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001516 Bees Insect members of the superfamily Apoidea, found almost everywhere, particularly on flowers. About 3500 species occur in North America. They differ from most WASPS in that their young are fed honey and pollen rather than animal food. Apidae,Apis,Apis mellifera,Apis mellifica,European Honey Bee,Honey Bee Drone,Bee,Bee, European Honey,Drone, Honey Bee,European Honey Bees,Honey Bee Drones,Honey Bee, European
D013754 Tetracyclines Closely congeneric derivatives of the polycyclic naphthacenecarboxamide. (Gilman et al., Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1117)
D052616 Solid Phase Extraction An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods. Extraction, Solid Phase,Extractions, Solid Phase,Solid Phase Extractions
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem

Related Publications

Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
June 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
January 2012, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
January 1998, Journal of capillary electrophoresis,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
December 2006, Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
January 2009, Journal of AOAC International,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
January 2004, Journal of agricultural and food chemistry,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
July 2010, Analytica chimica acta,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
October 2018, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
December 2006, Analytical and bioanalytical chemistry,
Maria Tarapoulouzi, and Christophoros Papachrysostomou, and Spyroula Constantinou, and Popi Kanari, and Militsa Hadjigeorgiou
March 2015, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Copied contents to your clipboard!