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Structure and gene cluster of the O-antigen of Escherichia coli O154. 2013
Andrei V Perepelov, and
Quan Wang, and
Sof'ya N Senchenkova, and
Lu Feng, and
Alexander S Shashkov, and
Lei Wang, and
Yuriy A Knirel
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. perepel@ioc.ac.ru
The O-polysaccharide (O-antigen) of Escherichia coli O154 was studied by sugar analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established: [structure: see text]. The O-antigen gene cluster of E. coli O154 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure.
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