Quantum dot-based immunochromatography test strip for rapid detection of Campylobacter jejuni. 2013

Feng Xu, and Xu Di, and Xing Ming, and Hengyi Xu, and Bo Li, and Peng Li, and Zoraida P Aguilar, and Tingtao Cheng, and Xiaoli Wu, and Hua Wei
Jiangxi-OAl Joint Research Institute, Nanchang University, Nanchang 330047, P R. China.

Campylobacter jejuni is a recognized human foodborne pathogen which is one of the leading causes of human gastrointestinal enteritis worldwide. In stress conditions, C. jejuni is able to enter into a viable but non-culturable state that is difficult to diagnose. Hence a rapid, sensitive, and specific method is required to monitor food and water for natural or intentional contamination by this pathogen. We report a quantum dot (QD)-based immunochromatography test strip (QDITS) for rapid detection of C. jejuni. The QDITS is based on a sandwich type immunoassay with QD fluorescence for sensitive detection. Under optimized conditions, this QD-based fluorescence biosensor exhibits detection of 10(4) CFU/ml in pure culture of C. jejuni which is 10 times more sensitive than colloidal-gold ITS. The improved sensitivity is attributed to the high quantum yield and brightness of the photostable QDs. When the QDITS were tested in 206 chicken samples and 120 beef samples, this exhibited a specificity of 98.5% and 99.1% respectively. On these real samples, the sensitivity of the QDITS was 100% in agreement with traditional culture method. The QDITS that took only 10 minutes to complete hold promise for rapid, sensitive detection of C. jejuni in real samples without the need for sample preparations steps.

UI MeSH Term Description Entries
D011934 Reagent Strips Narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances. (From Dorland, 28th ed) Reagent Strip,Strip, Reagent,Strips, Reagent
D002846 Chromatography, Affinity A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D001681 Biological Assay A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc. Bioassay,Assay, Biological,Assays, Biological,Biologic Assay,Biologic Assays,Assay, Biologic,Assays, Biologic,Bioassays,Biological Assays
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D015169 Colony Count, Microbial Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing. Agar Dilution Count,Colony-Forming Units Assay, Microbial,Fungal Count,Pour Plate Count,Spore Count,Spread Plate Count,Streak Plate Count,Colony Forming Units Assay, Microbial,Colony Forming Units Assays, Microbial,Agar Dilution Counts,Colony Counts, Microbial,Count, Agar Dilution,Count, Fungal,Count, Microbial Colony,Count, Pour Plate,Count, Spore,Count, Spread Plate,Count, Streak Plate,Counts, Agar Dilution,Counts, Fungal,Counts, Microbial Colony,Counts, Pour Plate,Counts, Spore,Counts, Spread Plate,Counts, Streak Plate,Dilution Count, Agar,Dilution Counts, Agar,Fungal Counts,Microbial Colony Count,Microbial Colony Counts,Pour Plate Counts,Spore Counts,Spread Plate Counts,Streak Plate Counts
D016123 Campylobacter jejuni A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals. Campylobacter fetus subsp. jejuni,Vibrio hepaticus,Vibrio jejuni
D045663 Quantum Dots Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES. Nanocrystals, Semiconductor,Semiconductor Nanocrystals,Semiconductor Nanoparticles,Dot, Quantum,Dots, Quantum,Nanocrystal, Semiconductor,Nanoparticle, Semiconductor,Nanoparticles, Semiconductor,Quantum Dot,Semiconductor Nanocrystal,Semiconductor Nanoparticle
D019544 Equipment Failure Analysis The evaluation of incidents involving the loss of function of a device. These evaluations are used for a variety of purposes such as to determine the failure rates, the causes of failures, costs of failures, and the reliability and maintainability of devices. Materials Failure Analysis,Prosthesis Failure Analysis,Analysis, Equipment Failure,Analysis, Materials Failure,Analysis, Prosthesis Failure,Analyses, Equipment Failure,Analyses, Materials Failure,Analyses, Prosthesis Failure,Equipment Failure Analyses,Failure Analyses, Equipment,Failure Analyses, Materials,Failure Analyses, Prosthesis,Failure Analysis, Equipment,Failure Analysis, Materials,Failure Analysis, Prosthesis,Materials Failure Analyses,Prosthesis Failure Analyses

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