BIOMAT Database Logo BIOMAT Database Logo

Determination of glucan phosphorylation using heteronuclear 1H, 13C double and 1H, 13C, 31P triple-resonance NMR spectra. 2013

Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, D-13125, Berlin, Germany.

Phosphorylation and dephosphorylation of starch and glycogen are important for their physicochemical properties and also their physiological functions. It is therefore desirable to reliably determine the phosphorylation sites. Heteronuclear multidimensional NMR-spectroscopy is in principle a straightforward analytical approach even for complex carbohydrate molecules. With heterogeneous samples from natural sources, however, the task becomes more difficult because a full assignment of the resonances of the carbohydrates is impossible to obtain. Here, we show that the combination of heteronuclear (1) H,(13) C and (1) H,(13) C,(31) P techniques and information derived from spectra of a set of reference compounds can lead to an unambiguous determination of the phosphorylation sites even in heterogeneous samples.

UI MeSH Term Description Entries
D009682 Magnetic Resonance Spectroscopy Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING). In Vivo NMR Spectroscopy,MR Spectroscopy,Magnetic Resonance,NMR Spectroscopy,NMR Spectroscopy, In Vivo,Nuclear Magnetic Resonance,Spectroscopy, Magnetic Resonance,Spectroscopy, NMR,Spectroscopy, Nuclear Magnetic Resonance,Magnetic Resonance Spectroscopies,Magnetic Resonance, Nuclear,NMR Spectroscopies,Resonance Spectroscopy, Magnetic,Resonance, Magnetic,Resonance, Nuclear Magnetic,Spectroscopies, NMR,Spectroscopy, MR
D010759 Phosphorus Isotopes Stable phosphorus atoms that have the same atomic number as the element phosphorus, but differ in atomic weight. P-31 is a stable phosphorus isotope. Isotopes, Phosphorus
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D011522 Protons Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion. Hydrogen Ions,Hydrogen Ion,Ion, Hydrogen,Ions, Hydrogen,Proton
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002247 Carbon Isotopes Stable carbon atoms that have the same atomic number as the element carbon but differ in atomic weight. C-13 is a stable carbon isotope. Carbon Isotope,Isotope, Carbon,Isotopes, Carbon
D005936 Glucans Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages. Glucan,Polyglucose,Polyglucoses,Glucan (BO),Glucose Polymer,Polycose,Polymer, Glucose

Related Publications

Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
Assignment of the side-chain 1H and 13C resonances of interleukin-1 beta using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopy.
September 1990, Biochemistry,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
1H, 15N, 13C, and 13CO assignments of human interleukin-4 using three-dimensional double- and triple-resonance heteronuclear magnetic resonance spectroscopy.
May 1992, Biochemistry,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
A novel approach for sequential assignment of 1H, 13C, and 15N spectra of proteins: heteronuclear triple-resonance three-dimensional NMR spectroscopy. Application to calmodulin.
May 1990, Biochemistry,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
Assignment of the aliphatic 1H and 13C resonances of the Bacillus subtilis glucose permease IIA domain using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopy.
May 1992, Biochemistry,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
1H- and 13C-NMR spectra of fenobam.
February 1983, Journal of pharmaceutical sciences,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
13C Satellite-Free 1H NMR Spectra.
November 2017, Analytical chemistry,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
1H, 13C and 15N NMR spectra of ciprofloxacin.
October 2004, Magnetic resonance in chemistry : MRC,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
Complete 1H and 13C NMR spectra of pregnenolone.
June 1995, Steroids,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
1H-, 13C- and 31P-NMR studies of dioctanoylphosphatidylcholine and dioctanoylthiophosphatidylcholine.
May 1990, Chemistry and physics of lipids,
Peter Schmieder, and Felix Nitschke, and Martin Steup, and Keven Mallow, and Edgar Specker
Synthesis and total assignment of 1H and 13C NMR spectra of new oxoisoaporphines by long-range heteronuclear correlations.
December 2005, Magnetic resonance in chemistry : MRC,
Copied contents to your clipboard!