Biomaterial Database

Easy handling of cell suspensions for electron microscopy. 1990

K Pihakaski-Maunsbach, and A Soitamo, and Suoranta U-M

Department of Biology, University of Turku, Finland.

We present here a new, simple agar encapsulating technique, which is helpful when preparing a small quantity of isolated fragile cells, e.g. protoplasts, for electron microscopy.

UI	MeSH Term	Description	Entries
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D011523	Protoplasts	The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.	Protoplast
D000362	Agar	A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
D012434	Secale	Genus in the grass family (Poaceae) including well known species Secale cereale (rye).	Rye,Secale cereale,Ryes
D013048	Specimen Handling	Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.	Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings