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The activity of purified bovine adrenocortical P-450(11)beta on the C18-steroid, 4-estrene-3,17-dione (19-norandrostenedione), is described. The major steroid products were separated by HPLC and identified by GC-MS, and 1H- and 13C-NMR as 11 beta-, 18- and 6 beta-hydroxylated derivatives of 19-norandrostenedione. The turnover numbers of the 11 beta-, 18- and 6 beta-hydroxylase reactions were 45, 7.5 and 1.9 (mol/min/mol of P-450(11)beta), respectively, with a common Km of 44 microM. All of these activities required the presence of the electron donating system consisting of NADPH, adrenal ferredoxin (adrenodoxin) and its reductase. These findings provide additional insights into the versatile catalytic roles of P-450(11)beta in the adrenal cortex, in which it may act on C18-19-nor-steroids in addition to its known activities on C21- and C19-steroids.
K Suhara, and
M Yamamoto, and
M Katagiri
November 1988,
Archives of biochemistry and biophysics,
K Suhara, and
M Yamamoto, and
M Katagiri
April 1969,
Biochemistry,
K Suhara, and
M Yamamoto, and
M Katagiri
July 1982,
Molecular and cellular endocrinology,
K Suhara, and
M Yamamoto, and
M Katagiri
September 1987,
Journal of biochemistry,
K Suhara, and
M Yamamoto, and
M Katagiri
November 1980,
Biochemical and biophysical research communications,
K Suhara, and
M Yamamoto, and
M Katagiri
November 1988,
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K Suhara, and
M Yamamoto, and
M Katagiri
August 1978,
Biochimica et biophysica acta,
K Suhara, and
M Yamamoto, and
M Katagiri
December 1976,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
K Suhara, and
M Yamamoto, and
M Katagiri
November 1974,
Life sciences,
K Suhara, and
M Yamamoto, and
M Katagiri
August 1957,
Nature,
