There is little information about the effects of short-term non-hypo-, and hyperfunction of teeth on the 1) mineralization patterns of intrinsic and extrinsic (Sharpey's) fibers and 2) mean number and diameter of Sharpey's fibers of adjacent alveolar bone. The mineral density of intrinsic and Sharpey's fibers and the size and number of Sharpey's fibers could indicate the relative strength of the attachment of a tooth to bone in various functional situations. In the present study, non- and hypofunctional situations were created by selective extraction of right molar teeth of the rat; the contralateral teeth were placed in hyperfunction by the surgery. In non- and hypofunctionals, intrinsic and Sharpey's fibers of the crestal third of the alveolus were less densely mineralized than in hyperfunctionals or untreated controls. Mean Sharpey's fiber diameters were significantly greater and their mean number/unit area significantly less in non- than in hypo- or hyperfunctionals or untreated controls (P less than 0.001). Mean Sharpey's fiber diameters in hyperfunctionals were significantly less than in untreated controls (P less than 0.05). Hypofunction ameliorated the effects of nonfunction on mean diameter and number of Sharpey's fibers, but had little effect on the density of mineralization of either the intrinsic or Sharpey's fibers of the alveolus, suggesting that their mineralization may be controlled by factors other than occlusal forces from the adjacent teeth. Thus changes in the stress/strain environment within the periodontium, coincident to altered occlusal function of the adjacent teeth, rapidly affects the morphology of intrinsic and Sharpey's fibers of alveolar bone and ensures that adequate tooth support is maintained in the new functional situation.