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An immunochemical comparison of components of cholesterol side chain cleavage system from bovine adrenocortical and human placental mitochondria has been carried out. Antibodies against cytochrome P-450scc, adrenodoxin reductase and adrenodoxin from bovine adrenocortical mitochondria were shown to cross-react with corresponding antigens of human placental mitochondria. A highly sensitive immunochemical method for cytochrome P-450scc determination has been developed. Limited proteolysis of cytochrome P-450scc of human placental mitochondria was studied, and the products of trypsinolysis were identified using antibodies against cytochrome P-450scc and fragments of its polypeptide chain: F1, F2 and F3. Immunochemical relatedness of ferredoxins from bovine adrenocortical and human placental mitochondria allowed one to develop a fast and efficient method for cytochrome P-450scc purification from human placental mitochondria by affinity chromatography on adrenodoxin-Sepharose.
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
November 1984,
Biokhimiia (Moscow, Russia),
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
April 1990,
Biokhimiia (Moscow, Russia),
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
January 1978,
Doklady Akademii nauk SSSR,
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
January 1984,
Bioorganicheskaia khimiia,
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
July 1982,
Biokhimiia (Moscow, Russia),
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
January 1985,
Bioorganicheskaia khimiia,
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
May 1978,
The Journal of biological chemistry,
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
January 1985,
Doklady Akademii nauk SSSR,
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
October 1987,
Biokhimiia (Moscow, Russia),
S A Usanov, and
A A Chernogolov, and
P Honkakoski, and
M Lang, and
M Passanen, and
H Raunio, and
O Pelkonen
August 1978,
Biochimica et biophysica acta,
