Two cases of visceral leishmaniasis with a species from Leishmania donovani complex were detected in the Fundeni Clinical Institute. In one case, two infection sources were possible: one from Italy, where the patient worked three years, the other from the Southwest of Romania (Dolj County), where he was resident and where few human and canine leishmaniasis cases were registered in the past. In the second case, the patient lived in the Northeast of Romania (Iaşi County), but worked in the same southwest zone. In both cases, a local transmission should be considered, situation that could amplify and extend in the future, supposing that increased temperatures will persist, favoring the persistence and multiplication of autochthonous and allochthonous Phlebotomus vector species.