-transparent.png){kind=logo}
Previous studies on gene expression profiles in primary biliary cirrhosis (PBC) have exclusively focused on liver tissue or intrahepatic cells. Since the pathological process is systemic, other complementary studies in blood cells seemed to be reasonable. In this research, we try to explore differentially expressed genes in peripheral blood mononuclear cells (PBMCs) of PBC patients. Nine PBC patients and 9 healthy controls were recruited as Cohort 1 for a microarray study of screening. Total RNA of PBMCs from each individual was isolated and screened by oligonucleotide microarray (22 K). Then, differentially expressed genes were categorized into signaling pathways. Expression levels of three important genes, tyrosine kinase binding protein (TYROBP), C–C motif chemokine 5 (CCL5) and cathepsin L (CTSL) were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) in a second Cohort 2 (30 PBC patients and 20 healthy controls). Results show that sixty-five genes differentially expressed in PBC were identified, 20 of which were up-regulated and 45 of which were down-regulated. Twenty-seven signaling pathways were identified. TYROBP and CCL5 were proved to be down-regulated in PBC, and CTSL was proved to be up-regulated (p < 0.05) in PBC, which were all consistent with the screening study. In conclusions, the analysis of gene expression in PBMCs of PBC and the comparison of gene profiles between PBMCs and the liver may provide new clues to the pathogenesis of the disease.
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
March 2013,
Journal of gastroenterology and hepatology,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
October 2014,
Hepatology research : the official journal of the Japan Society of Hepatology,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
April 2014,
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
August 2005,
World journal of gastroenterology,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
June 2013,
World journal of gastroenterology,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
May 2015,
The Journal of asthma : official journal of the Association for the Care of Asthma,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
January 2020,
Obesity facts,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
March 2006,
Proceedings of the National Academy of Sciences of the United States of America,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
September 2005,
The American journal of gastroenterology,
Lei Zhang, and
Danxu Ma, and
Xi Li, and
Chuiwen Deng, and
Qun Shi, and
Xin You, and
Xiaomei Leng, and
Mengtao Li, and
Fulin Tang, and
Fengchun Zhang, and
Yongzhe Li
August 2005,
Physiological genomics,
