Lymphatic transport of puerarin occurs after oral administration of different lipid-based formulations to unconscious lymph duct-cannulated rats. 2014

An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
Department of Pharmaceutics, Anhui University of Chinese Medicine , Anhui Province , China .

This study investigated the potential of targeted intestinal lymphatic transport of puerarin via a lipid formulation approach. Three formulations of PEG nanoemulsion, nanosuspension and an oil suspension containing puerarin were examined with the lymph-cannulated anaesthetized rat model. Plasma and lymph samples were analyzed by HPLC. Lymph triglyceride was measured using an enzymatic colorimetric technique. After 8 h, the total administered dose accumulated in the thoracic lymph duct was analyze. Nanoemulsion, nanosuspension and oil suspension was 0.065 ± 0.006%, 0.137 ± 0.018%, 0.021 ± 0.002% of the administered dose, respectively. In nanoemulsion, nanosuspension and oil suspension group, the systemic bioavailability of oral puerarin was 11%, 16% and 11% for lymph-cannulated rats, 41%, 67% and 18% for control rats. Absorption into the intestinal lymph should thus contribute to ∼30%, 51% and 7% of the systemic bioavailable puerarin. This data indicated that lipid-based nano drug formulation produced higher lymph concentrations of puerarin than oil suspension. The nanosuspension formulation may be considerable in terms of increased local concentrations in lymphoid tissue.

UI MeSH Term Description Entries
D007408 Intestinal Absorption Uptake of substances through the lining of the INTESTINES. Absorption, Intestinal
D007529 Isoflavones 3-Phenylchromones. Isomeric form of FLAVONOIDS in which the benzene group is attached to the 3 position of the benzopyran ring instead of the 2 position. 3-Benzylchroman-4-One,3-Benzylidene-4-Chromanone,Homoisoflavone,Homoisoflavones,Isoflavone,Isoflavone Derivative,3-Benzylchroman-4-Ones,3-Benzylidene-4-Chromanones,Isoflavone Derivatives,3 Benzylchroman 4 One,3 Benzylchroman 4 Ones,3 Benzylidene 4 Chromanone,3 Benzylidene 4 Chromanones,Derivative, Isoflavone,Derivatives, Isoflavone
D008055 Lipids A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed) Lipid
D008208 Lymphatic System A system of organs and tissues that process and transport immune cells and LYMPH. Lymphatic Systems
D008297 Male Males
D009821 Oils Unctuous combustible substances that are liquid or easily liquefiable on warming, and are soluble in ether but insoluble in water. Such substances, depending on their origin, are classified as animal, mineral, or vegetable oils. Depending on their behavior on heating, they are volatile or fixed. (Dorland, 28th ed)
D011092 Polyethylene Glycols Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS. Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D002626 Chemistry, Pharmaceutical Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use. Medicinal Chemistry,Chemistry, Pharmaceutic,Pharmaceutic Chemistry,Pharmaceutical Chemistry,Chemistry, Medicinal
D004655 Emulsions Colloids formed by the combination of two immiscible liquids such as oil and water. Lipid-in-water emulsions are usually liquid, like milk or lotion. Water-in-lipid emulsions tend to be creams. The formation of emulsions may be aided by amphiphatic molecules that surround one component of the system to form MICELLES. Emulsion
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations

Related Publications

An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
January 2016, Drug delivery,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
November 2014, Journal of agricultural and food chemistry,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
September 2003, Pharmaceutical research,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
November 2021, Lipids,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
October 2012, Cardiovascular drugs and therapy,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
August 2011, Lipids,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
March 2015, Journal of visualized experiments : JoVE,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
December 2001, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
August 2006, Pharmaceutical research,
An Zhou, and Tao Lu, and Lei Wang, and Chuanhua Lu, and Lina Wang, and Maolin Wan, and Hongfei Wu
September 2003, The Journal of pharmacology and experimental therapeutics,
Copied contents to your clipboard!