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The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum. 2013
Erzsébet Baka, and
Sándor Varga, and
Cédric Hobel, and
Roland Wilhelm Knispel, and
Csaba Fekete, and
Milán Ivanics, and
Balázs Kriszt, and
István Nagy, and
József Kukolya
Department of Environmental Protection & Environmental Safety, Szent István University, Gödöllő 2100, Hungary; Department of Microbiology, Central Environmental and Food Science Research Institute, Budapest 1022, Hungary.
A transformation method yielding up to 10(4) transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58°C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination.
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