-transparent.png){kind=logo}
Blood group A-active glycosphingolipids from human erythrocyte membranes were identified by the combination of thin-layer chromatography and matrix-assisted secondary ion mass spectrometry (TLC/SIMS). Partially purified lipid extracts were chromatographed by TLC and then blood group A-active glycolipids were detected by TLC-immunostaining assay using anti-A antibody. The parts of the plates which contained the same Rf area as anti-A positive spots were cut out and subjected to direct SIMS analysis. The TLC/SIMS spectra were quite similar to those obtained by ordinary SIMS. Detailed information, such as molecular weight, molecular species, ceramide portion, and oligosaccharide sequence, was obtained. Also, peracetylated blood group A-active glycolipids were analyzed in a similar manner. After the position of A-active glycolipids on a TLC plate was confirmed by in situ deacetylation and TLC-immunostaining, acetylated A-active glycolipids were also analyzed by the TLC/SIMS. Enhanced sensitivity was obtained with peracetylated glycolipids. Consequently, small amounts of unpurified bioactive glycolipids can be readily analyzed by TLC/SIMS.
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
June 1996,
Glycoconjugate journal,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
May 1976,
Chemistry and physics of lipids,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
November 1975,
Chemistry and physics of lipids,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
July 1987,
Biochimica et biophysica acta,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
January 1995,
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
January 1988,
Zeitschrift fur Rechtsmedizin. Journal of legal medicine,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
November 2007,
Lipids,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
January 1990,
Methods in enzymology,
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
January 2023,
Methods in molecular biology (Clifton, N.J.),
Y Kushi, and
K Ogura, and
C Rokukawa, and
S Handa
December 1987,
Transplantation proceedings,
