Biomaterial Database

[The identification of esterase D isoenzyme (EsD) phenotypes in the dental pulp by isoelectric focusing]. 1990

D De Leo, and F Tagliaro

Istituto di Medicina Legale, Università di Verona.

An identification method based on the isoelectrofocusing (IEF) assay of the EsD phenotypes in dental cavity pulp is reported. The IEF has been carried out on polyacrylamide gel (T = 5% and C = 3%), using ampholines ranging pH 4.5-5.4. A pre-focusing of 20 min at 2,000 V, 100 mA and 8 W was used; focusing conditions were: 2,000 V, unlimited current and W, for 75 min at 4 degrees C. Electropherograms were read under UV at 366 nm after reaction with 4-methylumbelliferyl acetate giving a fluorescent product. The content of a single dental cavity has been found to be the smallest amount of sample needed for the assay in alive subjects, excluding dental pathology which could interfered with the results. In bodies, excluding interferences from abnormal preservation conditions, a clear decrease in the reliability of the assay has been observed after the 6th day since the death. In any case, on the basis of the authors' experience, the dental pulp, as compared to blood, seems a preferable sample for identification purposes.

UI	MeSH Term	Description	Entries
D007525	Isoelectric Focusing	Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.	Electrofocusing,Focusing, Isoelectric
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D002265	Carboxylic Ester Hydrolases	Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.	Carboxylesterases,Ester Hydrolases, Carboxylic,Hydrolases, Carboxylic Ester
D003643	Death	Irreversible cessation of all bodily functions, manifested by absence of spontaneous breathing and total loss of cardiovascular and cerebral functions.	End Of Life,End-Of-Life,Near-Death Experience,Cardiac Death,Determination of Death,Death, Cardiac
D003782	Dental Pulp	A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)	Dental Pulps,Pulp, Dental,Pulps, Dental
D004588	Electrophoresis, Cellulose Acetate	Electrophoresis in which cellulose acetate is the diffusion medium.	Cellulose Acetate Electrophoreses,Cellulose Acetate Electrophoresis,Electrophoreses, Cellulose Acetate
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005553	Forensic Dentistry	The application of dental knowledge to questions of law.	Dentistry, Forensic
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man