-transparent.png){kind=logo}
Denaturant gradient gel-chromatography [Endo, S. et al. (1983) Anal. Biochem. 131, 108-120] has been used to follow renaturation of chicken cystatin and human stefin A. Slow gradient of GuHCl was applied to the size-exclusion Superose 12 column. The column was maintained at 5 degrees C by circulating water from a cryostate. When the denatured protein has been injected it started to refold on the column. By the simplified equation, according to [Hanai, R. et al. (1986) Biophys. Chem. 25, 27-36] approximate kinetics of folding was obtained. No intermediate species in folding could be detected from 6M to 2M GuHCl, only the close-to-denatured state and the close-to-native state.
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
January 1996,
Methods in molecular biology (Clifton, N.J.),
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
January 1990,
Computers in biology and medicine,
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
January 1990,
Rinsho byori. The Japanese journal of clinical pathology,
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
November 1984,
Journal of immunological methods,
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
January 1998,
Methods in molecular biology (Clifton, N.J.),
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
April 1986,
Biochimie,
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
June 1990,
FEMS microbiology letters,
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
January 2022,
Methods in molecular biology (Clifton, N.J.),
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
January 1987,
Leukemia research,
E Zerovnik, and
J Kos, and
B Lenarcic, and
V Turk
January 1988,
Preparative biochemistry,
