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Immunogold labelling was used to study the distribution of newly synthesized slow muscle myosin (SM) at the ultrastructural level as it replaced fast muscle myosin (FM) in rabbit muscles undergoing stimulation-induced type transformation. Control fast muscle was labelled strongly with antibody to FM and control slow muscle with antibody to SM; label was confined to the A-band. Well-defined differences in the distribution of label within the A-band suggested that the monoclonal antibodies used corresponded to epitopes on different parts of the myosin molecule; this was confirmed by Western blots of subfragments prepared from FM and SM. After 4 weeks of continuous stimulation at 10 Hz, fibres of the tibialis anterior muscle reacted with antibodies to both isoforms; after 6 weeks, labelling was obtained only with antibody to SM. After a 7-week period of stimulation and 3 further weeks of recovery, fibres again reacted with both antibodies. In all positively-labelled sections, the distribution of gold particles was characteristic of the antibody and independent of the origin or history of the fibres. This observation supports the conclusion that newly synthesized myosin is capable of being incorporated throughout the length and cross-section of the A-band.
L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
July 2003,
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L L Franchi, and
A Murdoch, and
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C N Mayne, and
L Elliott, and
S Salmons
May 2001,
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L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
January 1981,
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L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
August 1976,
FEBS letters,
L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
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L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
November 1985,
FEBS letters,
L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
April 1994,
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L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
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L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
June 1992,
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L L Franchi, and
A Murdoch, and
W E Brown, and
C N Mayne, and
L Elliott, and
S Salmons
September 1984,
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