Biomaterial Database

[Experimental autoimmune model of muscle-specific kinase specific myasthenia gravis in rats]. 2013

Yu Chen, and Hua-bing Wang, and Yong-jun Wang

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.

OBJECTIVE To establish the experimental autoimmune Lewis rat model of myasthenia gravis (EAMG) specific for muscle-specific kinase (MuSK). METHODS Lewis rats were immunized subcutaneously with a single injection of 200 µl of MuSK (50/100 µg) emulsified in complete Freund's adjuvant (CFA). And MuSK was expressed according to the murine gene of MuSK (AY360453). Control rats were immunized with CFA alone. All rats were observed every other day in a blinded fashion for fatigue characteristics. The clinical symptoms were graded between 0 and 3. And the clinical scores were recorded. The serum levels of anti-MuSK IgG at Days 7 and 57 were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS The clinical symptoms of myasthenia gravis (MG) appeared at Day 16 and peaked at Days 18-22. The serum levels of anti-MuSK IgG were markedly elevated in the experimental groups compared with the control group. CONCLUSIONS EAMG induced by MuSK is successfully established in rats. And this animal model may help us elucidate the pathogenesis of anti-MuSK myasthenia (AMM) and discover new drug therapy in the future.

UI	MeSH Term	Description	Entries
D011917	Rats, Inbred Lew	An inbred strain of rat that is used in BIOMEDICAL RESEARCH.	Rats, Inbred Lewis,Rats, Lew,Inbred Lew Rat,Inbred Lew Rats,Inbred Lewis Rats,Lew Rat,Lew Rat, Inbred,Lew Rats,Lew Rats, Inbred,Lewis Rats, Inbred,Rat, Inbred Lew,Rat, Lew
D011950	Receptors, Cholinergic	Cell surface proteins that bind acetylcholine with high affinity and trigger intracellular changes influencing the behavior of cells. Cholinergic receptors are divided into two major classes, muscarinic and nicotinic, based originally on their affinity for nicotine and muscarine. Each group is further subdivided based on pharmacology, location, mode of action, and/or molecular biology.	ACh Receptor,Acetylcholine Receptor,Acetylcholine Receptors,Cholinergic Receptor,Cholinergic Receptors,Cholinoceptive Sites,Cholinoceptor,Cholinoceptors,Receptors, Acetylcholine,ACh Receptors,Receptors, ACh,Receptor, ACh,Receptor, Acetylcholine,Receptor, Cholinergic,Sites, Cholinoceptive
D004195	Disease Models, Animal	Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases.	Animal Disease Model,Animal Disease Models,Disease Model, Animal
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001323	Autoantibodies	Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.	Autoantibody
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D020720	Myasthenia Gravis, Autoimmune, Experimental	Any autoimmune animal disease model used in the study of MYASTHENIA GRAVIS. Injection with purified neuromuscular junction acetylcholine receptor (AChR) (see RECEPTORS, CHOLINERGIC) components results in a myasthenic syndrome that has acute and chronic phases. The motor endplate pathology, loss of acetylcholine receptors, presence of circulating anti-AChR antibodies, and electrophysiologic changes make this condition virtually identical to human myasthenia gravis. Passive transfer of AChR antibodies or lymphocytes from afflicted animals to normals induces passive transfer experimental autoimmune myasthenia gravis. (From Joynt, Clinical Neurology, 1997, Ch 54, p3)	Myasthenia Gravis, Experimental Autoimmune,Passive Transfer Experimental Autoimmune Myasthenia Gravis,Autoimmune Experimental Myasthenia Gravis,Experimental Autoimmune Myasthenia Gravis, Passive Transfer,Experimental Myasthenia,Experimental Myasthenia Gravis,Myasthenia Gravis, Autoimmune Experimental,Experimental Myasthenias,Myasthenia Gravis, Experimental,Myasthenia, Experimental,Myasthenias, Experimental
D020794	Receptor Protein-Tyrosine Kinases	A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.	PTK Receptor,Receptors, Protein-Tyrosine Kinase,Tyrosine Kinase Linked Receptor,Tyrosine Kinase Linked Receptors,Tyrosine Kinase Receptor,Tyrosine Kinase Receptors,PTK Receptors,Protein-Tyrosine Kinase Receptor,Receptor Protein-Tyrosine Kinase,Kinase Receptor, Tyrosine,Kinase, Receptor Protein-Tyrosine,Kinases, Receptor Protein-Tyrosine,Protein-Tyrosine Kinase Receptors,Protein-Tyrosine Kinase, Receptor,Protein-Tyrosine Kinases, Receptor,Receptor Protein Tyrosine Kinase,Receptor Protein Tyrosine Kinases,Receptor, PTK,Receptor, Protein-Tyrosine Kinase,Receptor, Tyrosine Kinase,Receptors, PTK,Receptors, Protein Tyrosine Kinase