-transparent.png){kind=logo}
Burkholderia pseudomallei is a Gram-negative environmental bacterium found in tropical climates that causes melioidosis. Culture remains the diagnostic gold standard, but isolation of B. pseudomallei from heavily contaminated sites, such as fecal specimens, can be difficult. We recently reported that B. pseudomallei is capable of infecting the gastrointestinal tract of mice and suggested that the same may be true in humans. Thus, there is a strong need for new culture techniques to allow for efficient detection of B. pseudomallei in fecal and other specimens. We found that the addition of norfloxacin, ampicillin, and polymyxin B to Ashdown's medium (NAP-A) resulted in increased specificity without affecting the growth of 25 B. pseudomallei strains. Furthermore, recovery of B. pseudomallei from human clinical specimens was not affected by the three additional antibiotics. Therefore, we conclude that NAP-A medium provides a new tool for more sensitive isolation of B. pseudomallei from heavily contaminated sites.
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
June 2006,
Diagnostic microbiology and infectious disease,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
July 2003,
Journal of clinical microbiology,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
October 2005,
Journal of clinical microbiology,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
May 2019,
BMC veterinary research,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
April 1979,
Pathology,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
March 1957,
Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
April 1957,
American journal of clinical pathology,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
June 2011,
Transactions of the Royal Society of Tropical Medicine and Hygiene,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
May 1997,
Journal of applied microbiology,
Andrew Goodyear, and
Linda Strange, and
Drew A Rholl, and
Joy Silisouk, and
David A B Dance, and
Herbert P Schweizer, and
Steven Dow
September 1981,
Journal of clinical microbiology,
