Cloning and molecular characterization of a cDNA encoding a small GTPase from Hevea brasiliensis. 2013

H L Li, and D Guo, and W M Tian, and S Q Peng
Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, China.

Small GTPases play a critical role in the regulation of a range of cellular processes including growth, differentiation, and intracellular transportation. The cDNA encoding a small GTPase, designated as HbGTPase1, was isolated from Hevea brasiliensis. HbGTPase1 was 882 bp long containing a 612-bp open reading frame encoding a putative protein of 203 amino acids, flanked by an 83-bp 5'-untranslated region (UTR) and a 187-bp 3'-UTR. The predicted molecular mass of HbGTPase1 is 22.62 kDa, with an isoelectric point of 5.06. The HbGTPase1 protein was predicted to possess the conserved functional regions of the small GTPase superfamily of proteins. Quantitative polymerase chain reaction analysis revealed that HbGTPase1 was constitutively expressed in all tissues tested. HbGTPase1 transcripts accumulated at relatively low levels in the flower, latex, and leaves, while HbGTPase1 transcripts accumulated at relatively high levels in bark. Transcription of HbGTPase1 in the latex was induced by jasmonate.

UI MeSH Term Description Entries
D007840 Latex A milky, product excreted from the latex canals of a variety of plant species that contain cauotchouc. Latex is composed of 25-35% caoutchouc, 60-75% water, 2% protein, 2% resin, 1.5% sugar & 1% ash. RUBBER is made by the removal of water from latex.(From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed). Hevein proteins are responsible for LATEX HYPERSENSITIVITY. Latexes are used as inert vehicles to carry antibodies or antigens in LATEX FIXATION TESTS. Latices,Latice
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D018076 DNA, Complementary Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. Complementary DNA,cDNA,cDNA Probes,Probes, cDNA
D018506 Gene Expression Regulation, Plant Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D020559 Monomeric GTP-Binding Proteins A class of monomeric, low molecular weight (20-25 kDa) GTP-binding proteins that regulate a variety of intracellular processes. The GTP bound form of the protein is active and limited by its inherent GTPase activity, which is controlled by an array of GTPase activators, GDP dissociation inhibitors, and guanine nucleotide exchange factors. This enzyme was formerly listed as EC 3.6.1.47 G-Proteins, Monomeric,GTP-Binding Proteins, Monomeric,Monomeric G-Protein,Monomeric G-Proteins,Small G-Protein,Small G-Proteins,Small GTPase,Small GTPases,ras-Related GTP-Binding Protein,ras-Related GTPase,ras-Related GTPases,ras-Related G-Proteins,ras-Related GTP-Binding Proteins,G Proteins, Monomeric,G-Protein, Monomeric,G-Protein, Small,G-Proteins, Small,G-Proteins, ras-Related,GTP Binding Proteins, Monomeric,GTP-Binding Protein, ras-Related,GTP-Binding Proteins, ras-Related,GTPase, Small,GTPase, ras-Related,GTPases, Small,GTPases, ras-Related,Monomeric G Protein,Monomeric G Proteins,Monomeric GTP Binding Proteins,Protein, ras-Related GTP-Binding,Proteins, ras-Related GTP-Binding,Small G Protein,Small G Proteins,ras Related G Proteins,ras Related GTP Binding Protein,ras Related GTP Binding Proteins,ras Related GTPase,ras Related GTPases
D028482 Hevea A plant genus of the family EUPHORBIACEAE, order Euphorbiales, subclass Rosidae. Commercial natural RUBBER is mainly obtained from Hevea brasiliensis but also from some other plants. Hevea brasiliensis,Rubber Tree,Hevea brasilienses,Heveas,Rubber Trees,Tree, Rubber,Trees, Rubber,brasiliensis, Hevea

Related Publications

H L Li, and D Guo, and W M Tian, and S Q Peng
March 1996, Plant molecular biology,
H L Li, and D Guo, and W M Tian, and S Q Peng
March 2009, Bioscience, biotechnology, and biochemistry,
H L Li, and D Guo, and W M Tian, and S Q Peng
September 1991, Plant physiology,
H L Li, and D Guo, and W M Tian, and S Q Peng
April 2009, Plant physiology and biochemistry : PPB,
H L Li, and D Guo, and W M Tian, and S Q Peng
June 2004, Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology,
Copied contents to your clipboard!