BIOMAT Database Logo BIOMAT Database Logo

Alterations in phosphorylated CREB expression in different brain regions following short- and long-term morphine exposure: relationship to food intake. 2013

Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Division of Endocrinology, Metabolism and Molecular Medicine, Department of Medicine, Charles R. Drew University of Medicine and Science and UCLA School of Medicine, 1731 E. 120th. Street, Los Angeles, CA 90059, USA.

BACKGROUND Activation of the cyclic adenosine monophosphate (cAMP)/phosphorylated CREB (P-CREB) system in different brain regions has been implicated in mediating opioid tolerance and dependence, while alteration of this system in the lateral hypothalamus (LH) has been suggested to have a role in food intake and body weight. METHODS Given that opioids regulate food intake, we measured P-CREB in different brain regions in mice exposed to morphine treatments designed to induce different degrees of tolerance and dependence. RESULTS We found that a single morphine injection or daily morphine injections for 8 days did not influence P-CREB levels, while the escalating dose of morphine regimen raised P-CREB levels only in the ventral tegmental area (VTA). Chronic morphine pellet implantation for 7 days raised P-CREB levels in the LH, VTA, and dorsomedial nucleus of the hypothalamus (DM) but not in the nucleus accumbens and amygdala. Increased P-CREB levels in LH, VTA, and DM following 7-day treatment with morphine pellets and increased P-CREB levels in the VTA following escalating doses of morphine were associated with decreased food intake and body weight. CONCLUSIONS The morphine regulation of P-CREB may explain some of the physiological sequelae of opioid exposure including altered food intake and body weight.

UI MeSH Term Description Entries
D007279 Injections, Subcutaneous Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin. Subcutaneous Injections,Injection, Subcutaneous,Subcutaneous Injection
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D009020 Morphine The principal alkaloid in opium and the prototype opiate analgesic and narcotic. Morphine has widespread effects in the central nervous system and on smooth muscle. Morphine Sulfate,Duramorph,MS Contin,Morphia,Morphine Chloride,Morphine Sulfate (2:1), Anhydrous,Morphine Sulfate (2:1), Pentahydrate,Oramorph SR,SDZ 202-250,SDZ202-250,Chloride, Morphine,Contin, MS,SDZ 202 250,SDZ 202250,SDZ202 250,SDZ202250,Sulfate, Morphine
D009021 Morphine Dependence Strong dependence, both physiological and emotional, upon morphine. Morphine Abuse,Morphine Addiction,Abuse, Morphine,Addiction, Morphine,Dependence, Morphine
D009292 Narcotic Antagonists Agents inhibiting the effect of narcotics on the central nervous system. Competitive Opioid Antagonist,Narcotic Antagonist,Opioid Antagonist,Opioid Antagonists,Opioid Receptor Antagonist,Opioid Reversal Agent,Competitive Opioid Antagonists,Opioid Receptor Antagonists,Opioid Reversal Agents,Agent, Opioid Reversal,Agents, Opioid Reversal,Antagonist, Competitive Opioid,Antagonist, Narcotic,Antagonist, Opioid,Antagonist, Opioid Receptor,Antagonists, Competitive Opioid,Antagonists, Narcotic,Antagonists, Opioid,Antagonists, Opioid Receptor,Opioid Antagonist, Competitive,Opioid Antagonists, Competitive,Receptor Antagonist, Opioid,Receptor Antagonists, Opioid,Reversal Agent, Opioid,Reversal Agents, Opioid
D010766 Phosphorylation The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety. Phosphorylations
D001835 Body Weight The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms. Body Weights,Weight, Body,Weights, Body
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response

Related Publications

Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Effects of long-term exposure to delta9-THC on expression of cannabinoid receptor (CB1) mRNA in different rat brain regions.
November 1998, Brain research. Molecular brain research,
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Long-term administration of morphine specifically alters the level of protein expression in different brain regions and affects the redox state.
January 2024, Open life sciences,
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Peripheral signals conveying metabolic information to the brain: short-term and long-term regulation of food intake and energy homeostasis.
December 2001, Experimental biology and medicine (Maywood, N.J.),
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Histological alterations following short-term smokeless tobacco exposure in humans.
July 1998, Journal of periodontal research,
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Developmental lead exposure: behavioral alterations in the short and long term.
January 2001, Neurotoxicology and teratology,
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Alterations in the hippocampal phosphorylated CREB expression in drug state-dependent learning.
October 2015, Behavioural brain research,
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Long-term human exposure to lead from different media and intake pathways.
October 2010, The Science of the total environment,
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
The short- and long-term effects of food intake on thermogenesis.
January 2014, Temperature (Austin, Tex.),
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
[Oxidative phosphorylation in different regions of the rat brain following morphine administration].
January 1976, Farmakologiia i toksikologiia,
Xiuhai Ren, and Kabirullah Lutfy, and Michael Mangubat, and Monica G Ferrini, and Martin L Lee, and Yanjun Liu, and Theodore C Friedman
Short- and long-term effects of changes in pleasantness on food intake.
June 2000, Appetite,
Copied contents to your clipboard!