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A murine monoclonal antibody has been raised against a partially purified preparation of hepatic cytochrome P-448 (form c) from beta-naphthoflavone-treated rats. The monoclonal origin of the antibody was established by limiting dilution culture and isoelectricfocusing. The antibody has been designated 3/4/2. It reacts with apparently homogeneous cytochrome P-448 from rat liver in solid phase assay. It also cross reacts with a number of other cytochromes P-450, from rat and rabbit. In addition, a positive reaction was obtained with microsomal fractions from a variety of species, including man. None of the species tested was negative. The antibody does not react appreciably with purified haemoproteins other than cytochromes P-450. Antibody 3/4/2 is not inhibitory, either in reconstituted systems or with intact microsomal fraction. However, evidence was obtained that the antibody does cause some perturbation of the tertiary structure of the apoprotein at or near the haem.
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
March 1976,
The Journal of biological chemistry,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
January 1975,
Advances in experimental medicine and biology,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
January 1978,
Methods in enzymology,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
June 1975,
Biochemical and biophysical research communications,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
September 1985,
Biochemical and biophysical research communications,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
October 1981,
Life sciences,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
January 1980,
The Journal of biological chemistry,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
August 1981,
The Journal of biological chemistry,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
July 1984,
Biochemical pharmacology,
A R Boobis, and
J McQuade, and
D Sesardic, and
R T Robson, and
C Hayward, and
E A Lock, and
C R Elcombe, and
M S Rose, and
D S Davies
January 1994,
Postepy biochemii,
