Biomaterial Database

Detection of antigen-specific suppressor T cell factor by sandwich radioimmunoassay using two monoclonal antibodies with different specificities. 1985

N Suzuki, and T Saito, and T Tokuhisa, and M Taniguchi

We established a sandwich radioimmunoassay using two monoclonal antibodies specific for the distinct allotypic determinants on heavy and light chains of antigen-specific suppressor T cell factors (TsF). By use of this assay system, we confirmed our previous findings that the allotypic determinants (Ct) detected by BALB/c anti-CB-20 (7C5 and 7D1) were shared in various TsF with different antigen specificities, and also that the genes coding for the Ct determinants were indicated to be located on the right side of the Igh-V gene cluster, because the assay system equally detected either KLH-TsF or OVA-TsF from Ighb mice, such as C57BL/6, CB-20 and BAB-14, but not from BALB/c or C3H with other Igh allotypes. Furthermore, the radioimmunoassay, when used with two different anti-Ct (7C5 and 7D1), preferentially detected the extracted form of TsF, whereas the secreted TsF was easily detected by the combination of anti-Ct (7D1) and anti-I-Jb. In fact, the assay system defined two types of Ts hybridomas: one producing TsF in cytoplasm or on the membrane but not secreting and another secreting in the culture supernates. We also demonstrated that materials bound to the plate coated with anti-Ct antibody that had been incubated with different concentrations of cell-free extracts exhibited the dose-dependent suppression of antibody responses.

UI	MeSH Term	Description	Entries
D008222	Lymphokines	Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.	Lymphocyte Mediators,Mediators, Lymphocyte
D008815	Mice, Inbred Strains	Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.	Inbred Mouse Strains,Inbred Strain of Mice,Inbred Strain of Mouse,Inbred Strains of Mice,Mouse, Inbred Strain,Inbred Mouse Strain,Mouse Inbred Strain,Mouse Inbred Strains,Mouse Strain, Inbred,Mouse Strains, Inbred,Strain, Inbred Mouse,Strains, Inbred Mouse
D011863	Radioimmunoassay	Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.	Radioimmunoassays
D006825	Hybridomas	Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.	Hybridoma
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918	Antibody Specificity	The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.	Antibody Specificities,Specificities, Antibody,Specificity, Antibody
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D000941	Antigens	Substances that are recognized by the immune system and induce an immune reaction.	Antigen
D013491	Suppressor Factors, Immunologic	Proteins, protein complexes, or glycoproteins secreted by suppressor T-cells that inhibit either subsequent T-cells, B-cells, or other immunologic phenomena. Some of these factors have both histocompatibility (I-J) and antigen-specific domains which may be linked by disulfide bridges. They can be elicited by haptens or other antigens and may be mass-produced by hybridomas or monoclones in the laboratory.	Immunologic Suppressor Factors,Suppressor T-Cell Factors,T-Cell Suppressive Factors,T-Suppressor Factors,Factors, Immunologic Suppressor,Factors, T Suppressor,Suppressor Factor (SF4),T Cell Suppressor Factors,Factors, Suppressor T-Cell,Factors, T-Cell Suppressive,Factors, T-Suppressor,Suppressive Factors, T-Cell,Suppressor Factors, T,Suppressor T Cell Factors,T Cell Suppressive Factors,T Suppressor Factors,T-Cell Factors, Suppressor