BIOMAT Database Logo BIOMAT Database Logo

MicroRNA expression profiling of the porcine developing hypothalamus and pituitary tissue. 2013

Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
College of Animal Science, Zhejiang University, Hangzhou 310058, China. nyxu@zju.edu.cn.

MicroRNAs (miRNAs), a class of small non-coding RNA molecules, play important roles in gene expressions at transcriptional and post-transcriptional stages in mammalian brain. So far, a growing number of porcine miRNAs and their function have been identified, but little is known regarding the porcine developing hypothalamus and pituitary. In the present study, Solexa sequencing analysis showed 14,129,397 yielded reads, 6,680,678 of which were related to 674 unique miRNAs. After a microarray assay, we detected 175 unique miRNAs in the hypothalamus, including 136 previously known miRNAs and 39 novel candidates, while a total of 140 miRNAs, including 104 known and 36 new candidate miRNAs, were discovered in pituitary. More importantly, 37 and 30 differentially expressed miRNAs from several developmental stages of hypothalamus and pituitary were revealed, respectively. The 37 differentially expressed miRNAs in hypothalamus represented 6 different expression patterns, while the 30 differentially expressed miRNAs in pituitary represented 7 different expression patterns. To clarify potential target genes and specific functions of these differentially expressed miRNAs in hypothalamus and pituitary, TargetScan and Gorilla prediction tools were then applied. The current functional analysis showed that the differentially expressed miRNAs in hypothalamus and pituitary shared many biological processes, with the main differences being found in tissue-specific processes including: CDP-diacylglycerol biosynthetic/metabolic process; phosphatidic acid biosynthetic/metabolic process; energy reserve metabolic process for hypothalamus; adult behavior; sterol transport/homeostasis; and cholesterol/reverse cholesterol transport for pituitary. Overall, this study identified miRNA profiles and differentially expressed miRNAs among various developmental stages in hypothalamus and pituitary and indicated miRNA profiles change with age and brain location, enhancing our knowledge about spatial and temporal expressions of miRNAs in the porcine developing brain.

UI MeSH Term Description Entries
D007031 Hypothalamus Ventral part of the DIENCEPHALON extending from the region of the OPTIC CHIASM to the caudal border of the MAMMILLARY BODIES and forming the inferior and lateral walls of the THIRD VENTRICLE. Lamina Terminalis,Preoptico-Hypothalamic Area,Area, Preoptico-Hypothalamic,Areas, Preoptico-Hypothalamic,Preoptico Hypothalamic Area,Preoptico-Hypothalamic Areas
D010902 Pituitary Gland A small, unpaired gland situated in the SELLA TURCICA. It is connected to the HYPOTHALAMUS by a short stalk which is called the INFUNDIBULUM. Hypophysis,Hypothalamus, Infundibular,Infundibular Stalk,Infundibular Stem,Infundibulum (Hypophysis),Infundibulum, Hypophyseal,Pituitary Stalk,Hypophyseal Infundibulum,Hypophyseal Stalk,Hypophysis Cerebri,Infundibulum,Cerebri, Hypophysis,Cerebrus, Hypophysis,Gland, Pituitary,Glands, Pituitary,Hypophyseal Stalks,Hypophyses,Hypophysis Cerebrus,Infundibular Hypothalamus,Infundibular Stalks,Infundibulums,Pituitary Glands,Pituitary Stalks,Stalk, Hypophyseal,Stalk, Infundibular,Stalks, Hypophyseal,Stalks, Infundibular
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D059467 Transcriptome The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells. Transcriptomes,Gene Expression Profiles,Gene Expression Signatures,Transcriptome Profiles,Expression Profile, Gene,Expression Profiles, Gene,Expression Signature, Gene,Expression Signatures, Gene,Gene Expression Profile,Gene Expression Signature,Profile, Gene Expression,Profile, Transcriptome,Profiles, Gene Expression,Profiles, Transcriptome,Signature, Gene Expression,Signatures, Gene Expression,Transcriptome Profile
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays
D035683 MicroRNAs Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing. RNA, Small Temporal,Small Temporal RNA,miRNA,stRNA,Micro RNA,MicroRNA,Primary MicroRNA,Primary miRNA,miRNAs,pre-miRNA,pri-miRNA,MicroRNA, Primary,RNA, Micro,Temporal RNA, Small,miRNA, Primary,pre miRNA,pri miRNA

Related Publications

Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
MicroRNA expression profiling of the porcine developing brain.
January 2011, PloS one,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
Glutaminyl-cyclase expression in the bovine/porcine hypothalamus and pituitary.
June 1995, Journal of neuroendocrinology,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
microRNA expression profiling of the developing mouse heart.
November 2012, International journal of molecular medicine,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
MicroRNA expression profiling of the developing murine upper lip.
August 2014, Development, growth & differentiation,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
MicroRNA profiling in the mouse hypothalamus reveals oxytocin-regulating microRNA.
August 2013, Journal of neurochemistry,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
microRNA Expression Profiling of Propofol-Treated Developing Rat Hippocampal Astrocytes.
August 2015, DNA and cell biology,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
Developmental microRNA expression profiling of murine embryonic orofacial tissue.
July 2010, Birth defects research. Part A, Clinical and molecular teratology,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
microRNA expression profiling of heart tissue during fetal development.
May 2014, International journal of molecular medicine,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
Persistent expression of activated notch in the developing hypothalamus affects survival of pituitary progenitors and alters pituitary structure.
August 2015, Developmental dynamics : an official publication of the American Association of Anatomists,
Lifan Zhang, and Zhaowei Cai, and Shengjuan Wei, and Huiyun Zhou, and Hongmei Zhou, and Xiaoling Jiang, and Ningying Xu
Molecular characterization and temporal expression profiling of presenilins in the developing porcine brain.
September 2007, BMC neuroscience,
Copied contents to your clipboard!