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SJL/J mice were immunized with polyclonal rat Ig light chains of two allotypes and immune spleen cells were fused with P3-Ag8.653 myeloma cells. 17 cell populations producing antirat Ig AB were cloned. Four clones, 1G9, L3E8, L2B2 and L2C5 have been characterized in detail. All MABs are of the IgG1, kappa isotype. Using monoclonal rat kappa chains it was demonstrated that all the AB react with rat Ig kappa chains. Further localization of antigenic determinants was performed using isolated L chain C domain. It was shown that all MABs are directed against C domain epitopes. L2C5 MAB binds selectively to August rat L chains, thus showing specificity for Igk-1a determinants. The remaining three clones bind equally well to L chains of different allotypes, but 1G9 clone binds preferentially to isolated L chains as compared to intact IgG molecules.
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
January 1982,
Hybridoma,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
June 1983,
Journal of immunology (Baltimore, Md. : 1950),
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
January 1982,
Hybridoma,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
January 1983,
Immunogenetics,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
January 1983,
Immunogenetics,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
May 1986,
Molecular immunology,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
January 1989,
Molekuliarnaia biologiia,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
December 1979,
Molecular immunology,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
January 1995,
Immunogenetics,
V V Cherapakhin, and
A V Chervonsky, and
A V Filatov, and
O V Rokhlin
October 1983,
Tissue antigens,
