-transparent.png){kind=logo}
The highly reproducible histochemical localization of glial fibrillary acidic protein (GFAP)+ qualifies it as an important marker of astrocytes in both research and clinical applications. The primary objective of this study was to produce monoclonal antibodies having the advantage of invariant specificity, affinity, and titer to GFAP-specific epitopes of wide species distribution. We report here the characterization of four monoclonal antibodies that recognize the same or spatially close epitopes specific to GFAP. The epitope(s) detected has been phylogenetically conserved; human, bovine, ovine, canine, porcine, rabbit, guinea pig, rat, murine, and chicken brain homogenates all specifically absorb monoclonal antibody activity. Of importance to the routine application of these new anti-GFAP monoclonal antibodies is the demonstration here of the stability of the antigen-antibody interaction in normal, reactive, and neoplastic astrocytes of both rat and human origin following various methods of fixation.
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
July 1983,
Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
June 1986,
Journal of neuroimmunology,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
January 1984,
Journal of neurochemistry,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
January 1984,
Histochemistry,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
January 1984,
Acta cytologica,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
January 1989,
Acta cytologica,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
January 1986,
Acta cytologica,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
September 1985,
The Journal of comparative neurology,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
January 2018,
Frontiers in neurology,
C N Pegram, and
L F Eng, and
C J Wikstrand, and
R D McComb, and
Y L Lee, and
D D Bigner
January 2017,
Endokrynologia Polska,
