Isolation, culture, and characterization of primordial germ cells in Mongolian sheep. 2014

Chun Xia Liu, and Wen Long Wang, and Rui Yuan Zhao, and Hai Tao Wang, and Yi Yi Liu, and Shen Yuan Wang, and Huan Min Zhou
College of Life Science, Inner Mongolia Agricultural University, 306 Zhao wu da street, Hohhot, Inner Mongolia, 010018, China.

This study was performed to culture and preliminarily identify the primordial germ cells (PGCs) isolated from the genital ridge of the Mongolian sheep fetus. The growth characteristics of the sheep PGCs were detected in different culture systems such as culture media, resources, and state and passages of feeder cells. The obtained embryonic germ (EG) cells were identified by morphology, enzymology, and immunofluorescence. The results showed that the sheep EG cell colonies were ridgy, typically nest like, and compact, and had regular edges. Alkaline phosphatase staining reaction was weakly positive. EG cells expressed Kit, Rex-1, Nanog, and Oct-4. Immunofluorescence detection was weakly positive for Oct3/4, whereas positive for SSEA-1, SSEA-3, SSEA-4, TRA-1-61, and TRA-1-80.

UI MeSH Term Description Entries
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D005854 Germ Cells The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS. Gamete,Gametes,Germ-Line Cells,Germ Line,Cell, Germ,Cell, Germ-Line,Cells, Germ,Cells, Germ-Line,Germ Cell,Germ Line Cells,Germ Lines,Germ-Line Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D015295 Antigens, Tumor-Associated, Carbohydrate Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies. Antigens, Carbohydrate, Tumor-Associated,CA Antigens,Cancer-Associated Carbohydrate Antigens,Carbohydrate Antigens, Tumor-Associated,Tumor-Associated Carbohydrate Antigens,Antigen, Carcinoma-Associated,CA Antigen,CA(Oxford) Antigen,Carcinoma-Associated Antigen,Epitectin,Antigen, CA,Antigen, Carcinoma Associated,Antigens, CA,Antigens, Cancer-Associated Carbohydrate,Antigens, Tumor-Associated Carbohydrate,Cancer Associated Carbohydrate Antigens,Carbohydrate Antigens, Cancer-Associated,Carbohydrate Antigens, Tumor Associated,Carcinoma Associated Antigen,Tumor Associated Carbohydrate Antigens
D016256 Lewis X Antigen A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, Lewis X antigen is a stage-specific embryonic antigen. Antigens, CD15,CD15 Antigens,Le(X) Antigen,Leu-M1 Antigens,Lewis X Related Antigens,SSEA-1,SSEA-1 Determinant,Stage-Specific Embryonic Antigen-1,3 alpha-Fucosyl-N-Acetyl Lactosamine,CD15 Antigen,Galbeta(1-4)Fucalpha(1-3)GlcNAc,Hapten X,Lewis X Hapten,SSEA 1,3 alpha Fucosyl N Acetyl Lactosamine,Antigen, Lewis X,Embryonic Antigen-1, Stage-Specific,Leu M1 Antigens,SSEA 1 Determinant,Stage Specific Embryonic Antigen 1,X Antigen, Lewis,X Hapten, Lewis
D050814 Octamer Transcription Factor-3 An octamer transcription factor that is expressed primarily in totipotent embryonic STEM CELLS and GERM CELLS and is down-regulated during CELL DIFFERENTIATION. Oct-3 Transcription Factor,Transcription Factor Oct-3,Oct-4 Transcription Factor,Octamer-Binding Protein 4,POU Domain, Class 5, Transcription Factor 1,POU5F1 Transcription Factor,Transcription Factor Oct-4,Oct 3 Transcription Factor,Oct 4 Transcription Factor,Oct-3, Transcription Factor,Oct-4, Transcription Factor,Octamer Binding Protein 4,Octamer Transcription Factor 3,Transcription Factor Oct 3,Transcription Factor Oct 4,Transcription Factor, Oct-3,Transcription Factor, Oct-4,Transcription Factor, POU5F1,Transcription Factor-3, Octamer
D055746 Stage-Specific Embryonic Antigens Cell-surface molecules that exhibit lineage-restricted patterns of expression during EMBRYONIC DEVELOPMENT. The antigens are useful markers in the identification of EMBRYONIC STEM CELLS. SSEA Antigens,Stage-Specific Embryonic Antigen,Antigen, Stage-Specific Embryonic,Antigens, SSEA,Antigens, Stage-Specific Embryonic,Embryonic Antigen, Stage-Specific,Embryonic Antigens, Stage-Specific,Stage Specific Embryonic Antigen,Stage Specific Embryonic Antigens
D018929 Cell Culture Techniques Methods for maintaining or growing CELLS in vitro. Cell Culture,Cell Culture Technique,Cell Cultures,Culture Technique, Cell,Culture Techniques, Cell

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