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The effects of 5-AZA-2'-deoxycytidine (5-AZA-CdR) on the induction of morphological and biochemical differentiation and inhibition of DNA methylation of human HL-60 myeloid leukemic cells were investigated. 5-AZA-CdR at concentrations of 0.1-1 microgram ml-1 for 48-h exposure produced significant morphological differentiation of HL-60 leukemic cells to a more mature phenotype, augmented the cell surface marker (OKMI) for mature granulocytes/monocytes and also increased the superoxide anion production. Under these same conditions 5-AZA-CdR inhibited the synthesis of 5-methylcytosine in DNA suggesting that there is a correlation between the induction of differentiation and inhibition of DNA methylation. At concentrations of 5-AZA-CdR that inhibit DNA methylation there was a marked decrease in colony-formation suggesting that the antileukemic action of this analog is related to the methylation of DNA.
R L Momparler, and
J Bouchard, and
J Samson
January 1990,
Leukemia research,
R L Momparler, and
J Bouchard, and
J Samson
June 1992,
Anti-cancer drugs,
R L Momparler, and
J Bouchard, and
J Samson
January 2000,
Advances in experimental medicine and biology,
R L Momparler, and
J Bouchard, and
J Samson
October 1990,
Cancer letters,
R L Momparler, and
J Bouchard, and
J Samson
May 1993,
Leukemia,
R L Momparler, and
J Bouchard, and
J Samson
December 1986,
Biochemical and biophysical research communications,
R L Momparler, and
J Bouchard, and
J Samson
April 1994,
Anti-cancer drugs,
R L Momparler, and
J Bouchard, and
J Samson
August 1983,
Cancer research,
R L Momparler, and
J Bouchard, and
J Samson
May 1993,
Leukemia,
R L Momparler, and
J Bouchard, and
J Samson
January 2017,
Frontiers in oncology,
