-transparent.png){kind=logo}
When Saccharomyces cerevisiae SY15 rho° mutant cells grown in media stabilized with 10% sorbitol were suspended in 2% sorbitol solutions, 60-70% of the population did not lyse and became permeable to native high molecular weight DNA. Maximal incorporation of DNA to DNase resistant state was measured after 60 min of incubation in presence of 5 μg/ml DNA and 10 mM CaCl2. These results suggest that the fragile mutants might be tested as hosts for transformation of whole yeast cells.
| UI | MeSH Term | Description | Entries |
|---|
Related Publications
P V Venkov, and
V P Ivanov
February 1974,
Biochemical and biophysical research communications,
P V Venkov, and
V P Ivanov
July 1989,
FEMS microbiology letters,
P V Venkov, and
V P Ivanov
January 1990,
Yeast (Chichester, England),
P V Venkov, and
V P Ivanov
April 1989,
Yeast (Chichester, England),
P V Venkov, and
V P Ivanov
June 1991,
Journal of general microbiology,
P V Venkov, and
V P Ivanov
July 1982,
Archives of biochemistry and biophysics,
P V Venkov, and
V P Ivanov
February 1979,
Journal of general microbiology,
P V Venkov, and
V P Ivanov
September 1994,
European journal of biochemistry,
P V Venkov, and
V P Ivanov
October 1984,
Journal of bacteriology,
P V Venkov, and
V P Ivanov
October 1995,
Genetics,
