Biomaterial Database

In vivo effects of LPS on B lymphocyte subpopulations. Migration of marginal zone-lymphocytes and IgD-blast formation in the mouse spleen. 1985

P H Groeneveld, and T Erich, and G Kraal

The aim of the present study was to analyze the effect of LPS on the localization and differentiation of splenic B lymphocytes. Therefore, we used a double immunoperoxidase technique which enabled us to detect both the IgM+ IgD- marginal zone lymphocytes and the IgM+ IgD+ follicular lymphocytes in the same tissue section. Next to a dramatic disappearance of the predominantly IgM+ IgD- lymphocytes in the marginal zone shortly after an intravenous injection of LPS, an increased number of these cells could be found in the splenic follicles. The present results strongly suggest that the IgM+ IgD- cells in the splenic follicles represent immigrating marginal zone lymphocytes, and not differentiating follicular B cells, because no IgM+ IgD- cells could be observed in the follicles of draining lymph nodes shortly after a subcutaneous injection of a similar amount of LPS. These observations support the suggestion that LPS induces a migration of marginal zone lymphocytes into the follicles. The present results also showed the formation of IgD plasmablasts in the inner PALS and around the terminal arterioles of the spleen after LPS administration. The induction of IgD plasmablasts appeared to be a specific effect of LPS which may be related to its toxic properties.

UI	MeSH Term	Description	Entries
D007072	Immunoglobulin D	An immunoglobulin which accounts for less than 1% of plasma immunoglobulin. It is found on the membrane of many circulating B LYMPHOCYTES.	IgD,IgD1,IgD2
D007075	Immunoglobulin M	A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally was called a macroglobulin.	Gamma Globulin, 19S,IgM,IgM Antibody,IgM1,IgM2,19S Gamma Globulin,Antibody, IgM
D007275	Injections, Intravenous	Injections made into a vein for therapeutic or experimental purposes.	Intravenous Injections,Injection, Intravenous,Intravenous Injection
D007279	Injections, Subcutaneous	Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin.	Subcutaneous Injections,Injection, Subcutaneous,Subcutaneous Injection
D008070	Lipopolysaccharides	Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)	Lipopolysaccharide,Lipoglycans
D008198	Lymph Nodes	They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.	Lymph Node,Node, Lymph,Nodes, Lymph
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D010950	Plasma Cells	Specialized forms of antibody-producing B-LYMPHOCYTES. They synthesize and secrete immunoglobulin. They are found only in lymphoid organs and at sites of immune responses and normally do not circulate in the blood or lymph. (Rosen et al., Dictionary of Immunology, 1989, p169 & Abbas et al., Cellular and Molecular Immunology, 2d ed, p20)	Plasmacytes,Cell, Plasma,Cells, Plasma,Plasma Cell,Plasmacyte
D002465	Cell Movement	The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.	Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005260	Female		Females