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The composition of two protosubtilins -- proteolytic enzymes of the enzymes isolated from submerged cultures of two Bacillus subtilis strains was investigated. Each of the preparations contained two proteinases that differed in their pH optimum. Conditions of chromatographic separation of two proteinases on CM-52 cellulose were tested. With the use of specific inhibitors and specific substrates it has been shown that one of the proteinases belongs to metal enzymes and is inhibited by ethylene diamine tetracetate (EDTA). Another proteinase, which is probably serine proteinase, is inhibited by diazopropine fluorophosphate (DFP). The isoelectric point of neutral proteinase is 8.15-8.20.
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
December 1979,
Zeitschrift fur plastische Chirurgie,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
November 1970,
Schweizerische medizinische Wochenschrift,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
November 1968,
Archives of biochemistry and biophysics,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
October 1970,
Science (New York, N.Y.),
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
February 1972,
Acta allergologica,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
January 1970,
Acta tuberculosea et pneumologica Belgica,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
May 1971,
The Journal of biological chemistry,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
January 1981,
Mikrobiologicheskii zhurnal,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
January 1954,
Comptes rendus des travaux du Laboratoire Carlsberg. Serie chimique,
E A Shishkova, and
T Ia Rafalovskaia, and
L A Krutova, and
N B Popova, and
L I Oreshchenko
April 1966,
Archives of biochemistry and biophysics,
