-transparent.png){kind=logo}
Electrophysiological recordings were used to analyze single calcium channels in planar lipid bilayers after membranes from bovine cardiac sarcolemmal vesicles had been incorporated into the bilayer. In these cell-free conditions, channels in the bilayer showed unitary barium or calcium conductances, gating kinetics, and pharmacological responses that were similar to dihydropyridine-sensitive calcium channels in intact cells. The open channel current varied in a nonlinear manner with voltage under asymmetric (that is, physiological) ionic conditions. However, with identical solutions on both sides of the bilayer, the current-voltage relation was linear. In matched experiments, calcium channels from skeletal muscle T-tubules differed significantly from cardiac calcium channels in their conductance properties and gating kinetics.
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
June 1987,
The Journal of general physiology,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
March 1992,
Biochimica et biophysica acta,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
January 2004,
Parasitology,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
October 2006,
The journal of physical chemistry. B,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
January 1992,
Methods in enzymology,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
January 1999,
Methods in enzymology,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
January 1984,
Nature,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
July 2012,
Cell research,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
August 1998,
Molecular pharmacology,
R L Rosenberg, and
P Hess, and
J P Reeves, and
H Smilowitz, and
R W Tsien
July 1984,
Science (New York, N.Y.),
