Biomaterial Database

Non-pyramidal neurons in the guinea pig hippocampus. A combined Golgi-electron microscope study. 1986

M Schlander, and M Frotscher

Morphological characteristics of non-pyramidal neurons in the guinea pig hippocampus (regions CA1 and CA3) were analyzed by a correlated light and electron microscopic approach. Following Golgi impregnation, the cells were first studied under the light microscope and classified according to the location of their cell bodies and the distribution of their dendrites in the different hippocampal layers. Next, the Golgi impregnated non-pyramidal neurons were gold-toned and deimpregnated, allowing an electron microscopic analysis of the identified structures. With regard to cell body location and dendritic pattern, non-pyramidal cells are a rather heterogeneous group of neurons. Their perikarya were found in all hippocampal layers and their dendrites had a less regular orientation when compared to pyramidal neurons and granule cells. Two basic types, i.e., "vertical" and "horizontal" non-pyramidal neurons are described. Many cells were of an intermediate type with dendrites extending in all directions. Non-pyramidal cell dendrites were mostly devoid of spines but exhibited numerous varicosities. Non-pyramidal cell axons could sometimes be seen extending towards the pyramidal cell layer. A surprising uniformity was observed when the impregnated, identified non-pyramidal neurons were studied in the electron microscope. Their perikarya exhibited a well-developed endoplasmic reticulum and indented nuclei. Both the cell bodies and the varicose dendrites were densely covered with synaptic boutons which mainly formed asymmetric synaptic contacts. Only occasionally were symmetric synaptic contacts observed. Non-pyramidal cell dendrites extending into the stratum lucidum of CA3 were found to be contacted by the giant boutons of mossy fiber axons. In addition to synaptic contacts, the dendrites of gold-toned non-pyramidal neurons formed gap junctions with neighboring dendrites. The results are discussed in relation to recent immunocytochemical studies which have shown non-pyramidal neurons in the hippocampus to contain gamma-aminobutyric acid and/or various neuropeptides.

UI	MeSH Term	Description	Entries
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D008867	Microtomy	The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.	Thin Sectioning,Ultramicrotomy,Sectioning, Thin,Sectionings, Thin,Thin Sectionings
D009474	Neurons	The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.	Nerve Cells,Cell, Nerve,Cells, Nerve,Nerve Cell,Neuron
D011712	Pyramidal Tracts	Fibers that arise from cells within the cerebral cortex, pass through the medullary pyramid, and descend in the spinal cord. Many authorities say the pyramidal tracts include both the corticospinal and corticobulbar tracts.	Corticobulbar Tracts,Corticospinal Tracts,Decussation, Pyramidal,Corticobulbar Tract,Corticospinal Tract,Pyramidal Decussation,Pyramidal Tract,Tract, Corticobulbar,Tract, Corticospinal,Tract, Pyramidal,Tracts, Corticobulbar,Tracts, Corticospinal,Tracts, Pyramidal
D003712	Dendrites	Extensions of the nerve cell body. They are short and branched and receive stimuli from other NEURONS.	Dendrite
D006046	Gold	A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006624	Hippocampus	A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.	Ammon Horn,Cornu Ammonis,Hippocampal Formation,Subiculum,Ammon's Horn,Hippocampus Proper,Ammons Horn,Formation, Hippocampal,Formations, Hippocampal,Hippocampal Formations,Hippocampus Propers,Horn, Ammon,Horn, Ammon's,Proper, Hippocampus,Propers, Hippocampus,Subiculums
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013194	Staining and Labeling	The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.	Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings