Biomaterial Database

Effect of internal calcium concentration on calcium currents in rat sensory neurones. 1986

J L Dupont, and J L Bossu, and A Feltz

Using the patch-clamp technique in whole-cell configuration we have investigated the effect of increasing the internal calcium concentration (Cai) from below 10(-8) M to 10(-6) M on the three calcium currents: ICa,T (T for transient), ICa,S (S for sustained), ICa,N (N for neither), recently described in rat sensory neurones. Increasing Cai led to a dose-dependent reduction of the amplitude of ICa,S and, as Cai reached 5 X 10(-7) M ICa,S was nearly abolished. ICa,N is well evidenced from 5 X 10(-10) M to 10(-7) M where its is a large current. Preliminary observations indicate an increase of its inactivation rate following, as expected for a possible Cai dependent-inactivation, the increase of Cai from 5 X 10(-10) M to 10(-7) M. With Ca = 5 X 10(-7) M, all the cells displayed ICa,T and half of the cells in addition ICa,N, but it was of small amplitude. At Cai = 10(-6) M, most of the recorded cells only exhibited ICa,T.

UI	MeSH Term	Description	Entries
D007473	Ion Channels	Gated, ion-selective glycoproteins that traverse membranes. The stimulus for ION CHANNEL GATING can be due to a variety of stimuli such as LIGANDS, a TRANSMEMBRANE POTENTIAL DIFFERENCE, mechanical deformation or through INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS.	Membrane Channels,Ion Channel,Ionic Channel,Ionic Channels,Membrane Channel,Channel, Ion,Channel, Ionic,Channel, Membrane,Channels, Ion,Channels, Ionic,Channels, Membrane
D009475	Neurons, Afferent	Neurons which conduct NERVE IMPULSES to the CENTRAL NERVOUS SYSTEM.	Afferent Neurons,Afferent Neuron,Neuron, Afferent
D009994	Osmolar Concentration	The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.	Ionic Strength,Osmolality,Osmolarity,Concentration, Osmolar,Concentrations, Osmolar,Ionic Strengths,Osmolalities,Osmolar Concentrations,Osmolarities,Strength, Ionic,Strengths, Ionic
D002118	Calcium	A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.	Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D004563	Electrochemistry	The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.	Electrochemistries
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus