-transparent.png){kind=logo}
The absorbance changes of intrasynaptosomally entrapped arsenazo III have been converted into values of free Ca2+ concentration by correcting for the nonlinear response of arsenazo III at different concentrations of the dye as well as for changes in internal pH. An average resting value for free Ca2+ concentration around 0.4 microM is obtained. Depolarization with veratridine or gramicidin increases this value to around 3 microM. Measurements of cytosolic free Ca2+ with the quin2 method gives much lower values in similar conditions. The release of prelabelled [14C]noradrenaline from the nerve-endings is maximally activated when the internal free Ca2+ concentration rises as measured with arsenazo III to about 4 microM when titrated with increasing concentrations of ionophore A23187.
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
July 1982,
Neuroscience,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
January 1989,
Methods in enzymology,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
February 1987,
Biochemical and biophysical research communications,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
October 1981,
Biophysical journal,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
February 1988,
The American journal of physiology,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
January 1967,
Journal of electron microscopy,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
September 1990,
Regulatory peptides,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
November 1976,
Experientia,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
February 1984,
FEBS letters,
K E Akerman, and
E Heinonen, and
K Kaila, and
I G Scott
January 1983,
Nature,
