BIOMAT Database Logo BIOMAT Database Logo

Atomic force microscopic investigation of respiratory syncytial virus infection in HEp-2 cells. 2014

P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Center for NanoBiotechnology Research, Alabama State University, Montgomery, Alabama 36101, U.S.A.

Respiratory syncytial virus (RSV) primarily causes bronchiolitis and pneumonia in infants. In spite of intense research, no safe and effective vaccine has been developed yet. For understanding its pathogenesis and development of anti-RSV drugs/therapeutics, it is indispensable to study the RSV-host interaction. Although, there are limited studies using electron microscopy to elucidate the infection process of RSV, to our knowledge, no study has reported the morphological impact of RSV infection using atomic force microscopy. We report the cytoplasmic and nuclear changes in human epidermoid cell line type 2 using atomic force microscopy. Human epidermoid cell line type 2 cells, grown on cover slips, were infected with RSV and fixed after various time periods, processed and observed for morphological changes using atomic force microscopy. RSV infected cells showed loss of membrane integrity, with degeneration in the cellular content and cytoskeleton. Nuclear membrane was disintegrated and nuclear volume was decreased. The chromatin of the RSV infected cells was condensed, progressing towards degeneration via pyknosis and apoptosis. Membrane protrusions of ~150-200 nm diameter were observed on RSV infected cells after 6 h, suggestive of prospective RSV budding sites. To our knowledge, this is the first study of RSV infection process using atomic force microscopy. Such morphological studies could help explore viral infection process aiding the development of anti-RSV therapies.

UI MeSH Term Description Entries
D009685 Nuclear Envelope The membrane system of the CELL NUCLEUS that surrounds the nucleoplasm. It consists of two concentric membranes separated by the perinuclear space. The structures of the envelope where it opens to the cytoplasm are called the nuclear pores (NUCLEAR PORE). Nuclear Membrane,Envelope, Nuclear,Envelopes, Nuclear,Membrane, Nuclear,Membranes, Nuclear,Nuclear Envelopes,Nuclear Membranes
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002462 Cell Membrane The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells. Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D054884 Host-Pathogen Interactions The interactions between a host and a pathogen, usually resulting in disease. Host Pathogen Interaction,Host-Pathogen Relations,Pathogen-Host Interaction,Pathogen-Host Interactions,Host Pathogen Interactions,Host Pathogen Relations,Host-Pathogen Interaction,Host-Pathogen Relation,Interaction, Host Pathogen,Interaction, Host-Pathogen,Interaction, Pathogen-Host,Interactions, Host Pathogen,Interactions, Host-Pathogen,Interactions, Pathogen-Host,Pathogen Host Interaction,Pathogen Host Interactions,Pathogen Interaction, Host,Pathogen Interactions, Host,Relation, Host-Pathogen,Relations, Host-Pathogen
D018113 Respiratory Syncytial Virus, Human The type species of PNEUMOVIRUS and an important cause of lower respiratory disease in infants and young children. It frequently presents with bronchitis and bronchopneumonia and is further characterized by fever, cough, dyspnea, wheezing, and pallor. HRSV Human respiratory syncytial virus,Human respiratory syncytial virus,human RSV,RSV, human,human RSVs
D018625 Microscopy, Atomic Force A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample. Atomic Force Microscopy,Force Microscopy,Scanning Force Microscopy,Atomic Force Microscopies,Force Microscopies,Force Microscopies, Scanning,Force Microscopy, Scanning,Microscopies, Atomic Force,Microscopies, Force,Microscopies, Scanning Force,Microscopy, Force,Microscopy, Scanning Force,Scanning Force Microscopies

Related Publications

P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Inoculation of HEp-2 cells for respiratory syncytial virus isolation.
January 1983, Clinical pediatrics,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Improvement of respiratory syncytial virus replication in actively growing HEp-2 cells.
October 1983, Journal of virological methods,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
The cell to cell infection of respiratory syncytial virus in HEp-2 monolayer cultures.
July 1968, The Journal of general virology,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Calcium requirement for syncytium formation in HEp-2 cells by respiratory syncytial virus.
January 1988, Journal of clinical microbiology,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Maturation of respiratory syncytial virus within HEp-2 cell cytoplasm.
September 1996, Acta virologica,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Ultrastructural and Functional Characterization of Mitochondrial Dynamics Induced by Human Respiratory Syncytial Virus Infection in HEp-2 Cells.
July 2023, Viruses,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Infection with respiratory syncytial virus enhances expression of native receptors for non-pilate Neisseria meningitidis on HEp-2 cells.
February 1999, FEMS immunology and medical microbiology,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Comparison of immediate and delayed inoculation of HEp-2 cells for isolation of respiratory syncytial virus.
July 1984, Journal of clinical microbiology,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Real-time light scattering tracking of gold nanoparticles- bioconjugated respiratory syncytial virus infecting HEp-2 cells.
March 2014, Scientific reports,
P M Tiwari, and E Eroglu, and S Boyoglu-Barnum, and Q He, and G A Willing, and K Vig, and V A Dennis, and S R Singh
Prevalence of antibodies against HEp-2 cell antigen in infants and children hospitalized with respiratory syncytial virus infection.
January 1996, Infection,
Copied contents to your clipboard!