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Methods of preventing the degradation of 3H-methionine-enkephalin (3H-ME) in human blood both at 37 degrees C and under conditions of immediate cooling were examined. We found that, contrary to previous suggestions, use of aprotinin (with or without immediate cooling) was ineffective in preventing the degradation of 3H-ME in blood. Thus, previous reports on the circulating levels of ME which relied on such procedures to stabilize the ME may have reported artifactually low values. However, we found that citric acid effectively prevents 3H-ME breakdown in both human and rat blood. Thus, we propose the use of citric acid, mixed with blood immediately upon collection, as an effective method for the stabilization of ME in blood.
V J Aloyo, and
S A Mousa, and
G R Van Loon
January 1983,
Life sciences,
V J Aloyo, and
S A Mousa, and
G R Van Loon
January 1983,
The Journal of clinical endocrinology and metabolism,
V J Aloyo, and
S A Mousa, and
G R Van Loon
October 1984,
Acta endocrinologica,
V J Aloyo, and
S A Mousa, and
G R Van Loon
January 1983,
Life sciences,
V J Aloyo, and
S A Mousa, and
G R Van Loon
November 1982,
Neuroscience letters,
V J Aloyo, and
S A Mousa, and
G R Van Loon
March 1982,
The Journal of clinical investigation,
V J Aloyo, and
S A Mousa, and
G R Van Loon
August 1988,
International journal of peptide and protein research,
V J Aloyo, and
S A Mousa, and
G R Van Loon
January 1982,
Vestnik Akademii meditsinskikh nauk SSSR,
V J Aloyo, and
S A Mousa, and
G R Van Loon
August 1983,
Neuroscience letters,
V J Aloyo, and
S A Mousa, and
G R Van Loon
January 1989,
Peptides,
