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The species specificity of heat shock proteins of callus cultures of Nicotiana chinensis, Nicotiana glauca, Nicotiana tabacum, Atropa belladonna, Lycopersicon peruvianum, as well as some somatic hybrids of A. belladonna + N. chinensis, was investigated by means of polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Despite the general similarity of electrophoretic mobility of heat shock proteins from different plants, a number of species-specific differences between the proteins of distantly related genera were found. Heat shock proteins may thus serve as genetic markers in somatic hybridization.
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